Inhibition of Coronavirus Entry In Vitro and Ex Vivo by a Lipid-Conjugated Peptide Derived from the SARS-CoV-2 Spike Glycoprotein HRC Domain

MBio
Victor K OutlawMatteo Porotto

Abstract

The emergence of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the etiological agent of the 2019 coronavirus disease (COVID-19), has erupted into a global pandemic that has led to tens of millions of infections and hundreds of thousands of deaths worldwide. The development of therapeutics to treat infection or as prophylactics to halt viral transmission and spread is urgently needed. SARS-CoV-2 relies on structural rearrangements within a spike (S) glycoprotein to mediate fusion of the viral and host cell membranes. Here, we describe the development of a lipopeptide that is derived from the C-terminal heptad repeat (HRC) domain of SARS-CoV-2 S that potently inhibits infection by SARS-CoV-2. The lipopeptide inhibits cell-cell fusion mediated by SARS-CoV-2 S and blocks infection by live SARS-CoV-2 in Vero E6 cell monolayers more effectively than previously described lipopeptides. The SARS-CoV-2 lipopeptide exhibits broad-spectrum activity by inhibiting cell-cell fusion mediated by SARS-CoV-1 and Middle East respiratory syndrome coronavirus (MERS-CoV) and blocking infection by live MERS-CoV in cell monolayers. We also show that the SARS-CoV-2 HRC-derived lipopeptide potently blocks the spread of SARS-CoV-2 in ...Continue Reading

References

Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White
Jun 8, 2001·Annual Review of Biochemistry·D M Eckert, P S Kim
Mar 20, 2004·Nature Reviews. Drug Discovery·Tom MatthewsDani Bolognesi
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Jul 4, 2008·Nature Structural & Molecular Biology·Stephen C Harrison
Mar 5, 2009·The New England Journal of Medicine·Anne Moscona
Apr 2, 2010·Journal of Virology·Matteo PorottoAnne Moscona
Oct 11, 2013·Journal of Virology·Jeremy C WelschMatteo Porotto
Oct 19, 2013·Journal of Virological Methods·Xuefeng DengJianming Qiu
Dec 24, 2013·American Journal of Respiratory Cell and Molecular Biology·Andrea C SchambergerOliver Eickelberg
Feb 11, 2015·Antiviral Research·Yanchen ZhouGraham Simmons
Jan 20, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K LooiS M Stick
Jul 4, 2019·Journal of the American Chemical Society·Victor K OutlawAnne Moscona
Jan 18, 2020·The Journal of Biological Chemistry·Chanakha K NavaratnarajahRoberto Cattaneo
Feb 13, 2020·Cellular & Molecular Immunology·Shuai XiaLu Lu
Mar 11, 2020·Cell·Alexandra C WallsDavid Veesler
Mar 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shutoku MatsuyamaMakoto Takeda

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Citations

Nov 27, 2020·Microorganisms·Souvik Ghosh, Yashpal S Malik
Mar 8, 2021·Peptides·Hamid HeydariMehrdad Moosazadeh Moghaddam
Mar 22, 2021·Current Opinion in Virology·Gary R WhittakerJean K Millet
Mar 27, 2021·Ear, Nose, & Throat Journal·Gustavo Ferrer, Marcos A Sanchez-Gonzalez
Apr 16, 2021·Circulation Research·Mina K ChungJoseph Loscalzo
Jul 22, 2021·Biochemistry. Biokhimii︠a︡·Larisa V Kordyukova, Andrey V Shanko
Jul 29, 2021·Clinical Microbiology Reviews·Kaiming TaoRobert W Shafer

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Datasets Mentioned

BETA
PRJNA634194

Methods Mentioned

BETA
fluorescence microscopy
PCR
amidation
Chip
pulldown

Software Mentioned

bwa
LAVA
Geneious

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