Inhibition of coxsackievirus B4 replication in stably transfected cells expressing human MxA protein

Virology
V ChieuxD Hober

Abstract

Coxsackieviruses B (CVB) (B1-B6), positive-strand RNA viruses, cause a variety of diseases. CVB4 may have a causal role in insulin-dependent diabetes mellitus. IFN-alpha inhibits CVB replication; however, the mechanism is not well known. The interferon-alpha-inducible human MxA protein exerts an antiviral activity against negative-strand RNA viruses and against Semliki Forest virus, a positive-strand RNA virus. To test the antiviral spectrum of MxA against CVB4, we took advantage of stably transfected Vero cells expressing MxA (Vero/MxA) in 98% of cells. Compared with control cells, in Vero/MxA cells, CVB4 yields were dramatically reduced and expression of the VP1 CVB protein analyzed by immunofluorescence was highly restricted. Furthermore, the accumulation of positive- and negative-strand CVB4 RNA was prevented as shown by in situ hybridization and RT-PCR. These results indicate that the antiviral activity of MxA extends to CVB4 and that its replication cycle is inhibited at an early step in Vero/MxA cells.

References

Aug 1, 1991·Journal of Virology·P Staeheli, J Pavlovic
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M O DiazJ D Rowley
Jun 2, 1995·The Journal of Biological Chemistry·M SchwemmleP Staeheli
Jun 1, 1995·Journal of Virology·M FreseO Haller
Nov 1, 1994·Journal of Virology·S Schneider-SchauliesV ter Meulen
Mar 1, 1994·Fetal Diagnosis and Therapy·M DommerguesP Lebon
Aug 1, 1993·Clinical Immunology and Immunopathology·R KandolfA Heim
Apr 30, 1998·Journal of Virological Methods·V ChieuxP Wattré
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G Kochs, O Haller

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Citations

Mar 26, 2004·Nature Reviews. Immunology·Gregory A TaylorAlan Sher
Jun 26, 2008·Nature Reviews. Immunology·Anthony J Sadler, Bryan R G Williams
Oct 25, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Melissa M Brierley, Eleanor N Fish
Apr 7, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Michael LeroyDaniel Desmecht
Mar 6, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Martin Dermine, Daniel Desmecht
Mar 30, 2004·Journal of Virology·Ida AnderssonAli Mirazimi
Oct 27, 2006·Pediatric Research·Motokazu NakabayashiToshio Miyawaki
Dec 22, 2010·Medical Microbiology and Immunology·Nadine WiesenerAndreas Henke
Jun 16, 2007·Biochimie·Otto HallerGeorg Kochs
Apr 19, 2002·The Journal of General Virology·Sophie GirardBruno Blondel
Jul 30, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Balvinder K ManujaS K Gahlot
Jan 25, 2011·Virus Research·Yicheng ZhaoHongsheng Ouyang
Dec 31, 2003·Virology·Christopher Marlowe A CaipangTakashi Aoki
Jan 13, 2015·Trends in Microbiology·Otto HallerGeorg Kochs
May 29, 2015·Biotechnology, Biotechnological Equipment·Min Ji KimNam Joo Ha
Aug 19, 2016·BMC Veterinary Research·Yicheng ZhaoHongsheng Ouyang
Jul 14, 2004·Circulation·Raj DeonarainEleanor N Fish
May 10, 2013·Archives of Pharmacal Research·Do Kyung LeeNam Joo Ha
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zivana TezakLauren M Pachman
Jun 1, 2017·Journal of Medical Virology·Xuezhen ShiLimin Chen
Jan 7, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Todd M SchaeferCharles R Wira

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