Inhibition of cryoprecipitation of murine IgG3 anti-dinitrophenyl (DNP) monoclonal antibodies by anionic DNP-amino acid conjugates

European Journal of Immunology
F SpertiniS Izui

Abstract

Previously we have demonstrated that eight out of nine IgG3 monoclonal antibodies (mAb) obtained from autoimmune MRL-lpr/lpr mice were able to self-associate and to precipitate in the cold (Gyotoku et al., J. Immunol. 1987. 138:3785). To determine whether the cryoprecipitation of IgG3 mAb is enhanced or inhibited in the presence of specific ligand, we have established eight IgG3 mAb reactive with 2,4-dinitrophenol (DNP) hapten: four mAb were obtained from fusion of spleen cells of C57BL/6 mice immunized with 2,4,6-trinitrophenylated keyhole limpet hemocyanin, three from 129/Sv and one from BALB/c immunized with DNP-lipopolysaccharide. Five of them induced cryoglobulins composed exclusively of the IgG3 mAb. The binding of negatively charged monomeric DNP-amino acid conjugates completely inhibited the cryoprecipitation of all the five cryoprecipitating anti-DNP IgG3 mAb, while the incubation with positively charged or neutral DNP-amino acid conjugates had variable effects: increase, inhibition or no change of the cryoprecipitation. In addition, positively charged DNP-amino acid conjugates were able to induce the cryoprecipitation of one of the non-cryoprecipitating anti-DNP IgG3 mAb. Our data showed that (a) IgG3 mAb derived from...Continue Reading

References

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Citations

Oct 10, 2001·Springer Seminars in Immunopathology·Y PastoreS Izui
Jun 9, 2006·Bulletin of Experimental Biology and Medicine·N A Konstantinova, E N Karandashov
Jun 1, 1997·Molecular Immunology·D J Panka
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L ReiningerS Izui
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T FulpiusS Izui
Jan 18, 2012·Biology Direct·Neil S GreenspanSteven N Emancipator
Oct 2, 2012·Journal of the American Society of Nephrology : JASN·Masako OtaniShozo Izui
Jan 6, 2006·Kidney International·S H NasrV D D'Agati
Jan 1, 1992·Kidney International·R LemoineS Izui
Jan 1, 1995·European Journal of Immunology·D J PankaA Marshak-Rothstein
Feb 28, 2020·Annals of Hematology·Chao ZuoGaosi Xu
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuichi KikuchiShozo Izui
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiro KurodaShozo Izui
Feb 1, 1992·Clinical and Experimental Immunology·A M Denman

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