Abstract
The aim of this study was to investigate whether inhibition of connexin 43 gap junction-uncoupling is sufficient to prevent retinal vascular cell loss under high glucose condition and reduce cell monolayer permeability. Rat retinal endothelial cells were grown for 3, 5, and 7 days in normal (5 mM) or high glucose (30 mM) medium; in parallel, cells grown in high glucose medium were exposed for 3, 5, and 7 days to 100 nM danegaptide, which stabilizes connexin 43-mediated cell coupling. Additionally, cells grown in normal medium were treated with a connexin 43 blocker as a negative control. To determine gap junction intercellular communication, scrape load dye transfer assay was performed at the three time points. Cells were assessed for apoptosis and cell monolayer permeability by differential dye staining and in vitro permeability assays, respectively. Cells treated with danegaptide preserved gap junction intercellular communication, decreased cell death, and reduced cell monolayer permeability. Scrape load dye transfer assay indicated that cells exposed to danegaptide for 3, 5, and 7 days under high glucose condition maintained gap junction intercellular communication. Importantly, danegaptide significantly prevented high gluco...Continue Reading
Citations
Mar 1, 2020·Biomolecules·Moises Freitas-AndradeChristian C Naus
Aug 12, 2020·Visual Neuroscience·Cyril G EleftheriouBotir T Sagdullaev
Aug 16, 2019·Investigative Ophthalmology & Visual Science·Keith D RochfortPhilip M Cummins
Dec 22, 2020·Seizure : the Journal of the British Epilepsy Association·Ting-Ting YangFeng-Ru Tang
Nov 27, 2020·Chemico-biological Interactions·Chao LiZhongjun Du
Dec 6, 2020·Particle and Fibre Toxicology·Shuang LiangZhiwei Sun
Nov 25, 2020·Journal of Clinical Medicine·Dongjoon KimSayon Roy
Jan 12, 2021·Frontiers in Oncology·Kai-Jun LuoJean X Jiang
Apr 4, 2021·International Journal of Molecular Sciences·Jorge González-CasanovaDiana Rojas-Gomez
Apr 4, 2021·International Journal of Molecular Sciences·Paul E SquiresClaire E Hills
Dec 8, 2020·Experimental Eye Research·Henry H LouieOdunayo O Mugisho
May 19, 2021·Life Sciences·Ramkumar Katturajan, Sabina Evan Prince
Jul 3, 2021·Cells·Andrew M BoalDavid J Calkins
Jul 7, 2021·Biochimica Et Biophysica Acta. General Subjects·Guodong LiuFang Wang
Jun 27, 2020·The American Journal of Pathology·Yueru LiAndrius Kazlauskas