Inhibition of cytochrome c oxidase subunit 4 precursor processing by the hypoxia mimic cobalt chloride

Biochemical and Biophysical Research Communications
Eric HervouetCatherine Godinot

Abstract

Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the alpha subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected vhl. We show that, in addition to its effect on HIF-alpha subunits, CoCl2 prevented the normal processing of the precursor of cytochrome c oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences.

References

Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M GasserG Schatz
Aug 1, 1995·Nature Medicine·O IliopoulosW G Kaelin
Jun 30, 1994·The Science of the Total Environment·R Lauwerys, D Lison
Jul 11, 1998·European Journal of Biochemistry·L G NijtmansC Van den Bogert
Apr 20, 2001·Gene·M HüttemannL I Grossman
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Patrizia CavadiniGrazia Isaya
Aug 23, 2002·Biochimica Et Biophysica Acta·Oleksandr GakhGrazia Isaya
Aug 29, 2003·Biochimica Et Biophysica Acta·Petr PecinaJosef Houstek
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Nov 11, 2003·The Journal of Biological Chemistry·Siôn L WilliamsJan-Willem Taanman
Sep 18, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·A Vengellur, J J LaPres
Sep 28, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·William G Kaelin
Aug 9, 2005·The Biochemical Journal·Lukas StiburekJiri Zeman

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Citations

Dec 30, 2008·Journal of Industrial Microbiology & Biotechnology·Yuan LiNorio Sugiura
Mar 2, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Asne R BauskinSamuel N Breit
Jun 3, 2008·Carcinogenesis·Eric HervouetJosef Houstek
Aug 1, 2007·Journal of Bioenergetics and Biomembranes·Catherine GodinotHélène Simonnet
Mar 6, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Marcantonio BragadinSabrina Manente
Jun 17, 2014·Journal of Cellular Physiology·Pablo GarridoCelestino González
Apr 5, 2011·The Veterinary Journal·Carlos E Medina-TorresAli Mobasheri
Feb 8, 2008·Experimental Cell Research·Feng Qin LiuYan Chen
Oct 15, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Olga I KubrakVolodymyr I Lushchak
Aug 16, 2016·Free Radical Biology & Medicine·Thomas S Blacker, Michael R Duchen
Jan 24, 2007·Journal of Neuroimmunology·Antonella TacconelliAndrew R Mackay
Sep 28, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kathrin ChamaonChristoph H Lohmann
Sep 28, 2018·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Philippe Hantson
Nov 18, 2016·Circulation. Heart Failure·Milton Packer
Jul 6, 2014·Molecular Biology Reports·Nonkululeko N Mthembu, Lesetja Raymond Motadi

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