Inhibition of cytochrome P450 2B6 by Astragalus extract mixture HT042.

Toxicological Research
Harim KimDonghak Kim

Abstract

Astragalus extract mixture (AEM) HT042 is a functional food approved by the MFDS (Korean FDA) for increasing height. It comprises a mixture of three standardized extracts from Astragalus membranaceus root, Eleutherococcus senticosus stem, and Phlomis umbrosa root. In this study, drug-functional food interaction was analyzed using six major human cytochrome P450 enzymes. The inhibitory effect of AEM HT042 on P450 activities was studied using a P450-NADPH P450 reductase reconstitution system. Among the six P450 enzymes (1A2, 2A6, 2B6, 2D6, 2C9, and 3A4), only P450 2B6 activity was markedly decreased by AEM HT042 addition. The bupropion hydroxylation activity of P450 2B6 was analyzed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A calculated IC50 value of 10.62 µg/ml was obtained. To identify the inhibitory compounds in the mixture, four active compounds in AEM HT042 were analyzed. Shanzhiside methylester exhibited inhibitory effects on P450 2B6, whereas formononetin, eleutheroside E, and sesamoside did not affect P450 2B6 activity at all. Our results suggest that shanzhiside methylester in AEM HT042 is responsible for the inhibitory effect on P450 2B6 metabolism. Characterization of the inhi...Continue Reading

References

Aug 29, 1998·Archives of Biochemistry and Biophysics·T ShimadaE M Gillam
Aug 26, 1999·Drug Metabolism Reviews·S Ekins, S A Wrighton
Sep 2, 2000·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·J W BudzinskiJ T Arnason
Jun 18, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·B C FosterJ T Arnason
Nov 3, 2004·Archives of Biochemistry and Biophysics·Donghak Kim, F Peter Guengerich
Oct 7, 2005·The Journal of Biological Chemistry·Donghak KimF Peter Guengerich
Sep 11, 2008·Current Drug Metabolism·Hongbing Wang, Leslie M Tompkins
Aug 26, 2009·Current Drug Metabolism·Sui-Lin MoShu-Feng Zhou
May 4, 2010·Biochemical and Biophysical Research Communications·Hyoung-Goo ParkDonghak Kim
Nov 26, 2010·Journal of Medicinal Food·Mi-Yeon KimHocheol Kim
Mar 6, 2012·Phytotherapy Research : PTR·Mi-Yeon KimHocheol Kim
Jun 12, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hemali T AmunugamaPaul F Hollenberg
Oct 30, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sang Yoon LeeSang Kyum Kim
Nov 16, 2012·Drug Metabolism and Drug Interactions·Miia Turpeinen, Ulrich M Zanger
Mar 12, 2013·Methods in Molecular Biology·Cesar KenaanPaul F Hollenberg
Oct 31, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ji Young KimHocheol Kim
Dec 17, 2014·Chemico-biological Interactions·Jiaojiao CaoJiang Zheng
Jun 16, 2015·Chemical Research in Toxicology·Jaime D'AgostinoPaul F Hollenberg
Apr 17, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Matthew A Cerny
Jun 15, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Robert S Foti, Deepak K Dalvie
Jan 27, 2017·Current Drug Targets·Palrasu Manikandan, Siddavaram Nagini
Mar 10, 2017·Journal of Microbiology and Biotechnology·Hyoung-Goo ParkDonghak Kim
Jul 25, 2017·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jungbin SongHocheol Kim
Dec 11, 2017·Drug Metabolism and Pharmacokinetics·Dabin JeongDonghak Kim
May 4, 2018·Chemico-biological Interactions·Yejin LeeDonghak Kim

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