Inhibition of Cytochrome P450 Activities by Extracts of Hyptis verticillata Jacq.: Assessment for Potential HERB-Drug Interactions

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
David PickingR Delgoda

Abstract

Understanding the potential for adverse drug reactions (ADRs), from herb-drug interactions, is a key aspect of medicinal plant safety, with particular relevance for public health in countries where medicinal plant use is highly prevalent. We undertook an in-depth assessment of extracts of Hyptis verticillata Jacq., via its impact on activities of key cytochrome P450 (CYP) enzymes (CYPs 1A1, 1A2, 1B1, 3A4 and 2D6), its antioxidant properties (determined by DPPH assays) and chemical characterisation (using LC-MS). The dried plant aqueous extract demonstrated potent inhibition of the activities of CYPs 1A1 (7.6 µg/mL), 1A2 (1.9 µg/mL), 1B1 (9.4 µg/mL) and 3A4 (6.8 µg/mL). Further analysis of other crude extracts demonstrated potent inhibition of CYP1A2 activity for a dried plant ethanol extract (1.5 µg/mL), fresh plant ethanol extract (3.9 µg/mL), and moderate activity for a fresh plant aqueous extract (27.8 µg/mL). All four extracts demonstrated strong antioxidant activity, compared to the positive control (ascorbic acid, 1.3 µg/mL), with the dried plant ethanol extract being the most potent (1.6 µg/mL). Analysis of the dried plant aqueous extract confirmed the identity of seven phytochemicals, five lignans and two triterpenes. I...Continue Reading

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Citations

Aug 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Carla MarcotullioJudith X Becerra
Aug 9, 2020·Journal of Ethnopharmacology·Henrique BridiGilsane Lino von Poser
Jan 23, 2021·Pharmaceutics·Maria D AuxteroAna I Fernandes
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hassan SalhabJames Barker

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

BD Gentest
SigmaPlot
TRAMIL
Mass Lynx

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