Inhibition of DNA Methylation in Picochlorum soloecismus Alters Algae Productivity

Frontiers in Genetics
Christina R SteadmanScott N Twary

Abstract

Eukaryotic organisms regulate the organization, structure, and accessibility of their genomes through chromatin remodeling that can be inherited as epigenetic modifications. These DNA and histone protein modifications are ultimately responsible for an organism's molecular adaptation to the environment, resulting in distinctive phenotypes. Epigenetic manipulation of algae holds yet untapped potential for the optimization of biofuel production and bioproduct formation; however, epigenetic machinery and modes-of-action have not been well characterized in algae. We sought to determine the extent to which the biofuel platform species Picochlorum soloecismus utilizes DNA methylation to regulate its genome. We found candidate genes with domains for DNA methylation in the P. soloecismus genome. Whole-genome bisulfite sequencing revealed DNA methylation in all three cytosine contexts (CpG, CHH, and CHG). While global DNA methylation is low overall (∼1.15%), it occurs in appreciable quantities (12.1%) in CpG dinucleotides in a bimodal distribution in all genomic contexts, though terminators contain the greatest number of CpG sites per kilobase. The P. soloecismus genome becomes hypomethylated during the growth cycle in response to nitrog...Continue Reading

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Datasets Mentioned

BETA
GSE155500

Methods Mentioned

BETA
flow cytometry
ELISA
saturation binding
Bisulfite Sequencing
WGBS

Software Mentioned

AUGUSTUS
KofamKOALA
GitHub
GraphPad Prism
bismark bowtie2
MAKER
Illumina base calling
GraphPad
BLASTP
GraphPad Prism8

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