PMID: 9655174Jul 9, 1998Paper

Inhibition of DNA topoisomerase I activity by heparan sulfate and modulation by basic fibroblast growth factor

Molecular and Cellular Biochemistry
I KovalszkyR V Iozzo

Abstract

Eukaryotic DNA topoisomerase I catalyzes changes in the superhelical state of duplex DNA by transiently breaking single strands thereby allowing relaxation of both positively and negatively supercoiled DNA. Topoisomerase I is a nuclear enzyme localized at active sites of transcription, and abnormal levels of the enzyme have been observed in a variety of neoplasms. Because the enzyme binds heparin and, given the presence of heparan sulfate within the nuclei of mammalian cells, we sought to investigate the interaction between topoisomerase I and sulfated glycosaminoglycans isolated from normal and neoplastic human liver. The results demonstrated that low concentrations (approximately 100 nM) of heparan sulfate from normal liver but not from its malignant counterpart effectively blocked relaxation of supercoiled DNA driven by either purified holoenzyme or topoisomerase I activity present in nuclear extracts of three malignant cell lines. Heparin acted at even lower (approximately 10 nM) concentrations. Moreover, we show that basic fibroblast growth factor could interfere with this heparan sulfate/heparin-driven inhibition and that both basic fibroblast growth factor and heparin-binding sites co-localized in the nuclei of U937 leuk...Continue Reading

Citations

Nov 25, 2003·The Journal of Biological Chemistry·Jane ReilandDario Marchetti
Jul 20, 2007·Journal of Cellular and Molecular Medicine·Veronique Vreys, Guido David
Mar 24, 2009·PloS One·Ligong Chen, Ralph D Sanderson
Oct 14, 2014·BioMed Research International·József DudásIlona Kovalszky
Jul 24, 2020·PloS One·M KouwenbergJ van der Vlag
Aug 4, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Israel VlodavskyMichael Elkin
Feb 16, 2012·The FEBS Journal·Nikos AfratisNikos K Karamanos
Dec 9, 2017·BMC Cell Biology·Tünde SzatmáriKatalin Dobra
Mar 23, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shay Y SchubertOrit Goldshmidt
May 7, 2008·Journal of Cellular Biochemistry·Jo Ann Buczek-ThomasMatthew A Nugent
Feb 11, 2016·Marine Drugs·Matias J CardosoJoão F Mano
Jan 13, 2016·World Journal of Gastroenterology : WJG·Kornélia BaghyIlona Kovalszky
Nov 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cinzia Lanzi, Giuliana Cassinelli
Apr 24, 2001·The Tohoku Journal of Experimental Medicine·H MatsuyaM Endo
Aug 14, 1999·The Journal of Biological Chemistry·M Toyoshima, M Nakajima
Dec 16, 2010·Journal of Cellular and Molecular Medicine·Renato V Iozzo, Ralph D Sanderson
Jun 12, 2016·Biologie aujourd'hui·Sylvie Ricard-Blum, Frank Gondelaud
Oct 26, 2016·Oncology Reports·Kallirroi VoudouriGeorge N Tzanakakis
Jun 9, 2006·Differentiation; Research in Biological Diversity·Masahiko KobayashiNoriaki Tanaka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.