Inhibition of electrical coupling in pairs of murine pancreatic acinar cells by OAG and isolated protein kinase C

The Journal of Membrane Biology
R SomogyiH A Kolb


Gap junctional coupling was studied in pairs of murine pancreatic acinar cells using the double whole-cell patch-clamp technique. During stable electrical coupling, addition of OAG (1-oleoyl-2-acetyl-sn-glycerol) induced a progressive reduction of the junctional conductance to the detectable limit (approximately 3 pS). Prior to complete electrical uncoupling, various discrete single channel conductances between 20 and 100 pS could be observed. Polymyxin B, a potent inhibitor of the protein kinase C (PKC) system, completely suppressed OAG-stimulated electrical uncoupling. Dialysis of cell pairs with solutions containing PKC, isolated from rat brain, also caused electrical uncoupling. The presence of 0.1 mM dibutyryl cyclic AMP and 5 mM ATP in the pipette solution, which serves to stabilize the junctional conductance, did not suppress the effects of OAG or isolated PKC. We conclude that an increase of protein kinase C activity leads to the closure of gap junction channels, presumably via a PKC-dependent phosphorylation of the junctional peptide, and that this mechanism is dominant over cAMP-dependent upregulatory effects in the experimental time range (less than or equal to 1 hr). A correlation of the observed single channel cond...Continue Reading


Nov 28, 1979·Biochemical and Biophysical Research Communications·A W Murray, D J Fitzgerald
Aug 1, 1977·The Journal of Cell Biology·D L CasparW C Phillips
Aug 1, 1977·The Journal of Cell Biology·L MakowskiD A Goodenough
Oct 1, 1975·The Journal of General Physiology·J E Hall
Sep 2, 1985·Nature·J Neyton, A Trautmann
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G ZampighiM Kreman
Jan 1, 1985·The Journal of Membrane Biology·T YadaW R Loewenstein
Apr 15, 1986·European Journal of Biochemistry·P D LampeR G Johnson
Aug 29, 1986·Science·R D Veenstra, R L DeHaan
Apr 29, 1987·Biochemical and Biophysical Research Communications·T IshizukaY Nozawa
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P MedaL Orci
Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Pusch, E Neher
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Burt, D C Spray
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E M Lasater
Dec 1, 1987·The Journal of Cell Biology·E C BeyerD A Goodenough
Apr 14, 1986·Biochemical and Biophysical Research Communications·C W TaylorR P Rubin
Jan 5, 1987·FEBS Letters·A TakedaT Shimazu
Feb 15, 1985·Biochemical and Biophysical Research Communications·H S Gainer, A W Murray
Oct 1, 1988·Pflügers Archiv : European journal of physiology·C RandriamampitaA Trautmann
Jan 1, 1984·Journal of Pediatric Gastroenterology and Nutrition·D B Burnham, J A Williams
Jan 1, 1984·Annual Review of Physiology·B Sakmann, E Neher
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T EnomotoH Yamasaki
Feb 7, 1980·Nature·P N Unwin, G Zampighi


May 15, 1998·Journal of Cellular Biochemistry·R MassasD Benayahu
May 1, 1993·Pflügers Archiv : European journal of physiology·P N Münster, R Weingart
Nov 1, 1993·Molecular and Cellular Biochemistry·A J GodwinW H Fletcher
Sep 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·T L DyakonovaW Winlow
Apr 1, 1990·The Journal of Cell Biology·K Hyrc, B Rose
Jun 1, 1989·Journal of Protein Chemistry·R Somogyi, H A Kolb
Feb 1, 1992·Seminars in Cell Biology·J P RevelS B Yancey

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