PMID: 7024402Aug 1, 1981Paper

Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
B A Ponder, M M Wilkinson

Abstract

In mammals there are two forms of alkaline phosphatase, one of which is widely distributed in a variety of tissues, and one of which is confined to intestine. Levamisole (1-tetramisole) inhibits the nonintestinal form of the enzyme, but is without effect on the intestinal form. We have exploited this difference by using conjugates made with calf intestinal alkaline phosphatase for immunohistochemical demonstration of H2 antigens in frozen section of mouse tissues. The alkaline phosphatase staining is performed in the presence of 1 mm levamisole, which inhibits the endogenous tissue enzyme without loss of staining by the conjugate. Endogenous enzyme can be inhibited by other means, such as exposure to 20% acetic acid, but labile antigens may be destroyed.

Citations

Mar 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T P RothmanM D Gershon
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·J BerlingS von Kleist
Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·E RalfkiaerD Y Mason
May 1, 1986·The Histochemical Journal·B A PonderM M Wilkinson
Oct 1, 1985·Journal of Virological Methods·P H BergerT P Pirone
May 13, 1999·Progress in Histochemistry and Cytochemistry·K Ostergaard, D M Salter
Jul 4, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·A LiapisG Creatsas
Jan 24, 2002·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·C PerrinV Giordanengo
Aug 1, 1994·The Journal of Dermatologic Surgery and Oncology·C B ZacharyD A Kist
Jan 1, 1996·American Journal of Reproductive Immunology : AJRI·M H LachapelleD C Roy
Jun 1, 1985·Journal of Clinical Pathology·N FrickhofenH Heimpel
Jan 1, 1993·The Histochemical Journal·C M van der LoosJ J van den Oord
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T KirchnerH K Müller-Hermelink
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·K J HalbhuberN Zimmermann
Jun 1, 1986·The British Journal of Dermatology·E RalfkiaerD Y Mason

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.