PMID: 6167173May 1, 1981Paper

Inhibition of Entamoeba histolytica cytotoxin by alpha 1 antiprotease and alpha 2 macroglobulin

The American Journal of Tropical Medicine and Hygiene
W B LushbaughF E Pittman

Abstract

We previously reported partial purification of a proteinaceous substance with cytotoxic and enterotoxic activity isolated from the soluble fraction of sonicated axenically cultivated Entamoeba histolytica trophozoites. Demonstration of cytotoxic activity of the preparation (amebal toxin) was dependent on removal of serum from the tissue culture assay system. The objective of the present study was to identify the factor(s) in non-immune sera responsible for producing in vitro inhibition of amebal toxin cytotoxicity on HeLa cells. Gel filtration of non-immune sera from adult humans or bovines demonstrated that two portions of the eluate had significant inhibitory against the toxin. A high molecular weight inhibitory fraction was identified as predominantly alpha-2 macroglobulin and a low molecular weight inhibitory fraction was identified as predominantly alpha-1 antiprotease. Preparative isoelectric focusing of human serum isolated inhibitory fractions containing these same alpha globulins. Alpha-2 macroglobulin was purified and alpha-1 antiprotease was partially purified from human serum by other methods and shown to have high inhibitory activity against the amebal cytotoxin. Substances that were inhibitory to the cytotoxic act...Continue Reading

Citations

Feb 1, 1985·The Journal of protozoology·E E AvilaJ Calderón
Feb 22, 2012·Archivum Immunologiae Et Therapiae Experimentalis·David A BerginEmer P Reeves
Feb 1, 1985·The Journal of protozoology·A Martínez-PalomoA Cervantes
Sep 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·B D Mata-CárdenasS Said-Fernández
Nov 1, 1987·Molecular and Biochemical Parasitology·R Perez-MontfortI Becker
Aug 1, 1985·Journal of Clinical Microbiology·G A GentryW Lushbaugh
Jun 1, 1985·Experimental Parasitology·W B LushbaughF E Pittman
Jun 1, 1986·Prostaglandins, Leukotrienes, and Medicine·M Alam

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