Inhibition of enterovirus 71 (EV-71) infections by a novel antiviral peptide derived from EV-71 capsid protein VP1.

PloS One
Chee Wah TanChit Laa Poh

Abstract

Enterovirus 71 (EV-71) is the main causative agent of hand, foot and mouth disease (HFMD). In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers) overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD) cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC(50) values ranging from 6-9.3 µM in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against ...Continue Reading

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Citations

Feb 14, 2014·Journal of Biomedical Science·Chee Wah TanYoke Fun Chan
Jun 12, 2013·Expert Review of Anti-infective Therapy·Chee Wah Tan, Yoke-Fun Chan
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Jul 17, 2020·Nanomedicine·Hamza Elsayed Ahmed MohamedMalik Maaza
Mar 27, 2020·Virusdisease·Meng WangShasha Wang
Apr 16, 2020·BMC Complementary Medicine and Therapies·Salima S LalaniChit Laa Poh
Jan 17, 2021·Journal of Biomedical Science·Mohd Ishtiaq AnasirChit Laa Poh
Jul 28, 2021·Virus Research·Faisal ZarifChit Laa Poh

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Datasets Mentioned

BETA
AF316321
AM396586
AB204852
AM396584
AAK13008

Methods Mentioned

BETA
Assay
PCR
protein assay
Fluorescence

Software Mentioned

Cellomics HCS ArrayScan
Clustal W2

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