PMID: 9539106Apr 16, 1998Paper

Inhibition of epidermal growth factor-mediated ERK1/2 activation by in situ electroporation of nonpermeant [(alkylamino)methyl]acrylophenone derivatives

DNA and Cell Biology
H L BrownellL Raptis

Abstract

The interruption of signaling cascades in intact cells through the introduction of nonpermeant compounds inferred by in vitro studies to specifically inhibit epidermal growth factor (EGF) receptor (EGF-R) function is described. Two nonpermeant [(alkylamino)methyl]acrylophenone derivatives, [(dimethylamino)methyl] acrylo-para-[(benzoylsulfonyl)-oxy]phenone and [(dimethylamino)-methyl]acrylo-para-[(hydroxy-benzoylsulfonyl++ +)-oxy]phenone, were introduced by in situ electroporation into mouse or rat fibroblasts growing on indium-tin oxide-coated glass. Cells were subsequently stimulated with growth factors and assessed for activation of a downstream target, the extracellular signal-regulated kinase (ERK1/2), by probing with specific antibodies. Electrodes and slides were configured to provide non-electroporated control cells side by side with the electroporated ones, both growing on the same type of indium-tin oxide-coated glass surface. Using this set-up, these compounds could inhibit EGF- but not platelet-derived growth factor (PDGF)-mediated ERK1/2 activation in vivo. These results demonstrate the potential of the in situ electroporation approach for the study of tyrosine kinase action using selective but nonpermeant inhibitor...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Levitzki
Oct 1, 1990·DNA and Cell Biology·L Raptis, K L Firth
Sep 14, 1990·Biochemical and Biophysical Research Communications·A B Bahnson, S S Boggs
Oct 1, 1989·Journal of Medicinal Chemistry·A GazitA Levitzki
Jan 1, 1994·Annual Review of Cell Biology·P van der GeerR A Lindberg
Jul 8, 1994·Journal of Medicinal Chemistry·M P MaguireA Zilberstein
Oct 1, 1993·Journal of Medicinal Chemistry·M S SmythT R Burke
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Feb 19, 1993·Journal of Medicinal Chemistry·T R BurkeI D Horak
Jan 1, 1997·DNA and Cell Biology·H L BrownellL Raptis
Mar 1, 1997·Molecular and Cellular Biology·S Giorgetti-PeraldiS E Shoelson
Apr 1, 1997·Current Opinion in Cell Biology·M J Robinson, M H Cobb

❮ Previous
Next ❯

Citations

Jul 21, 1999·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·E TomaiL Raptis
Aug 28, 2003·DNA and Cell Biology·Evangelia TomaiLeda Raptis
Mar 29, 2014·Cancers·Stephanie GuyLeda Raptis
Feb 15, 2005·Analytical Biochemistry·R ArulanandamL Raptis
May 6, 2003·Analytical Biochemistry·Leda RaptisKevin L Firth
Dec 27, 2005·Cancer Letters·Aikaterini AnagnostopoulouLeda Raptis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.