Inhibition of erythrocyte sickling in vitro by pyridoxal

The Journal of Clinical Investigation
J A KarkL S Lessin

Abstract

To test the antisickling activity of pyridoxal, we compared the oxygen affinity and the percent sickling at low PO2 of untreated erythrocytes with values for cells from the same blood sample incubated with pyridoxal, glyceraldehyde, or pyridoxine. Pyridoxal increased oxygen affinity much more than glyceraldehyde. 20 mM pyridoxal and glyceraldehyde had equivalent antisickling activity. At PO2 levels above 20 mm Hg, both agents reduced sickling to less than 2%. In samples examined by electron microscopy, pyridoxal reduced the percent sickled cells and the percent cells that contain hemoglobin S fibers by the same amount (from 74 to 3%). Pyridoxine had no effect on oxygen affinity or sockling. Pyridoxal reacts with intracellular hemoglobin to increase oxygen affinity, which inhibits hemoglobin S polymerization and sickling.

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Nigen, J M Manning
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R BeneschT Suzuki
Oct 5, 1977·Journal of Molecular Biology·A ArnoneR Benesch
Jan 1, 1978·The Journal of Clinical Investigation·A M Nigen, J M Manning
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R BeneschS Yung
Apr 1, 1974·Archives of Internal Medicine·J G White
Dec 10, 1968·The Journal of Vitaminology·K Yamada, M Tsuji
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

❮ Previous
Next ❯

Citations

Sep 10, 2002·Journal of Pediatric Hematology/oncology·Melissa C NelsonVirginia A Stallings
May 1, 1983·The Journal of Clinical Investigation·J A KarkR Bongiovanni
Sep 1, 1987·Baillière's Clinical Haematology·J Stuart, C S Johnson
Nov 22, 2016·Journal of the Science of Food and Agriculture·Suprama DattaUday S Annapure
Feb 1, 1982·Biochemical Medicine·J A KarkR D Reynolds

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.