Inhibition of experimental autoimmune myocarditis: peripheral deletion of TcR Vbeta 8.1, 8.2+ CD4+ T cells in TLR-4 deficient mice.

Journal of Autoimmunity
Patricia A GonnellaFrancis X McGowan

Abstract

Toll-like receptors (TLR) are pattern recognition receptors that are an essential feature of host defense against pathogens. Expression of TLR-4 on dendritic cells was reported to be required for initiation of experimental autoimmune myocarditis (EAM) but the mechanism by which TLR-4 signaling affects autoimmunity is incompletely understood. To determine the role of TLR-4 in EAM, wild type and TLR-4-/- mice were immunized with myosin peptide (614-629) in CFA. TLR-4-/- mice demonstrated decreased myosin specific proliferation and decreased production of INF-gamma and IL-2. Immunization with myosin induced greater severity of myocarditis in wild type compared to TLR-4-/- mice as evidenced by lesions in the myocardium. TcR Vbeta 8.1, 8.2+ CD4+ T cells, detected in lesions were isolated from splenocytes by flow cytometry and found to undergo increased apoptosis in TLR-4-/- mice. In situ immunohistochemistry showed increased colocalization of cleaved caspase 3 and TcR Vbeta 8.1, 8.2+ CD4+ T cells in TLR-4-/- mice compared to wild type. Increased apoptosis was associated with impaired activation of NF-kB p65 and decreased cell viability in the presence of TNF-alpha. These results demonstrate that infiltrating TcR Vbeta 8.1, 8.2+ CD4+...Continue Reading

References

Feb 24, 1995·Cell·D Thanos, T Maniatis
Aug 1, 1993·Clinical Immunology and Immunopathology·N NeuP Berger
May 1, 1996·The Journal of Clinical Investigation·C L PummererN Neu
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·S GerondakisR J Grumont
Mar 24, 2004·Molecular Immunology·Madeleine W Cunningham
Sep 24, 2004·Reviews in Medical Virology·DeLisa FairweatherNoel R Rose
Jun 22, 2005·Cellular and Molecular Life Sciences : CMLS·S KodamaD L Faustman
Jan 13, 2006·Circulation·René R MartyUrs Eriksson
Aug 2, 2006·The Journal of Experimental Medicine·Manu RangachariUrs Eriksson
Oct 14, 2006·European Journal of Immunology·Ivo SondereggerManfred Kopf

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Citations

Sep 12, 2012·Journal of Neuropathology and Experimental Neurology·Anna BrunnMartina Deckert
Mar 10, 2011·Expert Opinion on Therapeutic Targets·Ulrich HofmannStefan Frantz
Aug 15, 2009·Journal of Autoimmunity·Florian LeuschnerZiya Kaya
Sep 18, 2009·Annals of the New York Academy of Sciences·Carlo Selmi
Dec 25, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Norihiko AshigakiMitsuaki Isobe
Jul 24, 2021·International Immunopharmacology·Zhang PingLiang Shao

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