Inhibition of fatty-acid synthase suppresses P-AKT and induces apoptosis in bladder cancer

Urology
Bo JiangShu-Jie Xia

Abstract

To investigate the role of fatty acid synthase (FASN) in bladder transitional cell carcinoma (BTCC). FASN expression was investigated in non-muscle-invasive BTCC tissue specimens by immunohistochemistry and BTCC cell lines by Western blot. After treatment with FASN-siRNA or FASN inhibitor cerulenin (Cer), the proliferation and apoptosis of BTCC cell lines 5637 and 253 J were determined by cell counting Kit-8 (CCK8) assay and flow cytometry respectively. The expression of p-AKT, cyclin D1 (CCND1), and apoptosis-related proteins were detected by Western blot. High levels of FASN expression were observed in 59% (32/54) of non-muscle-invasive BTCC tissue specimens, and FASN expression was associated with histologic grade (P < .05) and recurrence (P < .05). FASN expression was high in 6 BTCC cell lines. FASN inhibitor Cer and FASN-siRNA produced the increased apoptosis and decreased proliferation of bladder cancer cells, and caused inactivity of AKT and downregulation of CCND1. Furthermore, treatment of BTCC cell lines with Cer resulted in apoptosis via the caspase-dependent pathway involving inactivation of antiapoptotic bcl-2 protein. Our data suggest that FASN plays an important role in BTCC development. Targeting FASN may be a n...Continue Reading

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Citations

Mar 15, 2016·Cancer Treatment Reviews·Francesco MassariRodolfo Montironi
Apr 15, 2015·Prostate Cancer and Prostatic Diseases·J ChengJ R Marshall
Feb 6, 2018·Molecular and Cellular Biochemistry·M PreslerJ Swierczynski
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Sep 28, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Milad AshrafizadehRajender S Varma

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