PMID: 109429Jun 25, 1979

Inhibition of fatty acid synthesis prevents the incorporation of aldosterone-induced proteins into membranes.

The Journal of Biological Chemistry
W N ScottD B Goodman


2-Methyl-2-[p-(1,2,3,4-tetrahydro-1-naphthyl)phenoxy]propionic acid (TPIA), an acetyl coenzyme A carboxylase inhibitor, blocks the aldosterone-induced increase in transepithelial sodium transport. To examine the requirement for ongoing fatty acid synthesis and/or elongation in the aldosterone-induced alteration of cellular protein metabolism in the toad's urinary bladder, the effect of TPIA has been examined in double-labeled amino acid incorporation experiments. TPIA itself has no effect on the pattern of protein labeling in either the "soluble" or a plasma membrane-enriched fraction. However, inhibition of fatty acid synthesis selectively inhibits the aldosterone-induced incorporation of membrane proteins without altering the labeling of soluble cell protein. These results indicate that ongoing fatty acid synthesis is required for the hormone-induced changes in plasma membrane protein metabolism.

Related Concepts

Aldosterone, (11 beta,17 alpha)-Isomer
Amino Acids
Urinary Bladder
Bufo marinus
Plasma Membrane
Saturated Fat
Cell Surface Proteins

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