Apr 25, 1981

Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate

The Journal of Biological Chemistry
S J PilkisT Claus

Abstract

Rat liver fructose-1,6-bisphosphatase, which was assayed by measuring the release of 32P from fructose 1,6-[1-32P]bisphosphate at pH 7.5, exhibited hyperbolic kinetics with regard to its substrate. beta-D-Fructose 2,6-bisphosphate, an activator of hepatic phosphofructokinase, was found to be a potent inhibitor of the enzyme. The inhibition was competitive in nature and the Ki was estimated to be 0.5 microM. The Hill coefficient for the reaction was 1.0 in the presence and absence of fructose 2,6-bisphosphate. Fructose 2,6-bisphosphate also enhanced inhibition of the enzyme by the allosteric inhibitor AMP. The possible role of fructose 2,6-bisphosphate in the regulation of substrate cycling at the fructose-1,6-bisphosphatase step is discussed.

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Mentioned in this Paper

Fructose
Fructose 2,6-diphosphate
Fructose-1,6-Bisphosphatase
Hepatic
Phosphofructokinase
Pfkp
Fbp1
Pfkl
Metabolic Inhibition
Phosphaden

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