PMID: 7542774Jul 18, 1995Paper

Inhibition of function in Xenopus oocytes of the inwardly rectifying G-protein-activated atrial K channel (GIRK1) by overexpression of a membrane-attached form of the C-terminal tail

Proceedings of the National Academy of Sciences of the United States of America
N DascalN Davidson

Abstract

Coexpression in Xenopus oocytes of the inwardly rectifying guanine nucleotide binding (G)-protein-gated K channel GIRK1 with a myristoylated modification of the (putative) cytosolic C-terminal tail [GIRK1 aa 183-501 fused in-frame to aa 1-15 of p60src and denoted src+ (183-501)] leads to a high degree of inhibition of the inward G-protein-gated K+ current. The nonmyristoylated segment, src- (183-501), is not active. Although some interference with assembly is not precluded, the evidence indicates that the main mechanism of inhibition is interference with functional activation of the channel by G proteins. In part, the tail functions as a blocking particle similar to a "Shaker ball"; it may also function by competing for the available supply of free G beta gamma liberated by hormone activation of a seven-helix receptor. The non-G-protein-gated weak inward rectifier ROMK1 is less effectively inhibited, and a Shaker K channel was not inhibited. Immunological assays show the presence of a high concentration of src+ (183-501) in the plasma membrane and the absence of any membrane forms for the nonmyristoylated segment.

References

Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·N DascalD Trollinger

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Citations

Jan 16, 1998·Cellular Signalling·N Dascal
Aug 5, 1998·The Journal of General Physiology·T T Ivanova-NikolovaJ D Robishaw
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·D E Clapham, E J Neer
Aug 5, 2000·Biochemical and Biophysical Research Communications·T Keren-RaifmanN Dascal
Jan 28, 2003·Biophysical Journal·Carmen MüllnerWolfgang Schreibmayer
Jun 4, 2004·The Journal of Biological Chemistry·Sergej MilovicLukas G Weigl
Aug 25, 2000·The Journal of Biological Chemistry·M K LancasterM R Boyett
Apr 18, 1997·The Journal of Biological Chemistry·R WoodwardR D Murrell-Lagnado
Mar 7, 1997·The Journal of Biological Chemistry·K W ChanD E Logothetis
Feb 25, 2005·The Journal of Biological Chemistry·Ida RishalNathan Dascal
Sep 2, 1998·The American Journal of Physiology·A PleumsamranD Kim

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