PMID: 8971033Dec 1, 1996Paper

Inhibition of generation of authentic genomic termini of herpes simplex virus type 1 DNA in temperature-sensitive mutant BHK-21 cells with a mutated CCG1/TAF(II)250 gene

Journal of Virology
K Umene, T Nishimoto

Abstract

A temperature-sensitive (ts) mutant from the BHK-21 hamster cell line, tsBN462, has a defect in progression of the G1 phase at the nonpermissive temperature of 39.5 degrees C. The ts mutation in tsBN462 is located in the CCG1 gene, encoding the general transcription factor TAF(II)250. In tsBN462 at 39.5 degrees C, infectious progeny of herpes simplex virus type 1 (HSV-1) was not produced and generation of authentic genomic termini of HSV-1 was inhibited. HSV-1 concatemers containing L components in two possible orientations were produced in tsBN462 at 39.5 degrees C; hence, the generation of authentic genomic termini seemed to be dispensable for inversion of the L component. As production of mRNAs of HSV-1 genes of three kinetic classes in the tsBN462 at 39.5 degrees C was comparable to findings under permissive conditions, the sequential and regulated manner in which HSV-1 gene expression is processed is likely to be maintained in the nonpermissive condition.

References

Oct 1, 1990·The Journal of General Virology·C AddisonV G Preston
Jul 1, 1988·The Journal of General Virology·D J McGeochP Taylor
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R LongneckerB Roizman
May 1, 1983·Cell·J W SzostakF W Stahl
Sep 1, 1994·Journal of Virology·W W NewcombJ C Brown

❮ Previous
Next ❯

Citations

Sep 10, 1999·Reviews in Medical Virology·K Umene

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.