Inhibition of glutathione S-transferases by thonningianin A, isolated from the African medicinal herb, Thonningia sanguinea, in vitro

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Maxwell Afari GyamfiY Aniya

Abstract

There is evidence that increased expression of glutathione S-transferase (EC: 2.5.1.18, GST) is involved in resistance of tumor cells against chemotherapeutic agents. In this study we investigated the inhibitory effects of thonningianin A (Th A), a novel antioxidant isolated from the medicinal herb, Thonningia sanguinea on uncharacterized rat liver GST and human GST P1-1. Using 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, rat liver cytosolic GST activity was inhibited by Th A in a concentration dependent manner with 50% inhibition concentration (IC50) of 1.1 microM. When Th A was compared with known potent GST inhibitors the order of inhibition was tannic acid>cibacron blue>hematin>Th A>ethacrynic acid with CDNB as substrate. Th A also exhibited non-competitive inhibition towards both CDNB and glutathione. Furthermore, using 1,2-dichloro-4-nitrobenzene, ethacrynic acid and 1,2-epoxy-3-(p-nitrophenoxy) propane as substrates Th A at 1.0 microM inhibited cytosolic GST by 2%, 12% and 36% respectively. Human GST P1-1 was also inhibited by Th A with an IC50 of 3.6 microM. While Th A showed competitive inhibition towards CDNB it exhibited non-competitive inhibition towards GSH of the human GST P1-1. These results suggest that Th A...Continue Reading

References

Mar 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T D Boyer
Jan 1, 1989·Advances in Cancer Research·K Sato
Aug 1, 1988·Trends in Pharmacological Sciences·P J van Bladeren
Dec 30, 1986·Biochemical and Biophysical Research Communications·M DasY C Awasthi
Jun 15, 1985·Biochemical Pharmacology·J T AhokasB T Emmerson
Apr 1, 1974·The Biochemical Journal·E Haslam
Apr 30, 1984·Biochemical and Biophysical Research Communications·M DasH Mukhtar
Feb 1, 1980·European Journal of Biochemistry·R MorgensternL Ernster
Jul 28, 1995·International Journal of Cancer. Journal International Du Cancer·S BennisJ Robert
Jun 1, 1994·Biochemical Pharmacology·K Zhang, N P Das
Mar 16, 1994·Biochimica Et Biophysica Acta·M C Wilce, M W Parker
Jan 1, 1994·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A R BartholomaeusJ T Ahokas
Jul 15, 1993·The Biochemical Journal·D J MeyerB Ketterer
Nov 4, 1996·International Journal of Cancer. Journal International Du Cancer·S AwasthiY C Awasthi
Feb 1, 1996·Journal of Natural Products·E Haslam
Jan 1, 1997·Chemical Research in Toxicology·R N Armstrong
Jan 22, 1998·Archives of Biochemistry and Biophysics·P Jemth, B Mannervik
May 23, 1998·Annual Review of Pharmacology and Toxicology·M W Anders, W Dekant
Jan 5, 1999·The Journal of Biological Chemistry·B AlvarezR Radi
Feb 13, 2001·Biochemical and Biophysical Research Communications·R I van HaaftenA Bast
Jul 14, 2001·Biochimica Et Biophysica Acta·R I Van HaaftenA Bast
Oct 12, 2001·Archives of Biochemistry and Biophysics·P S WongA van der Vliet
Oct 20, 2001·Chemico-biological Interactions·R I van HaaftenA Bast
Jan 25, 2002·International Journal of Cancer. Journal International Du Cancer·Stanley MukanganyamaJulia A Hasler
May 15, 2002·Biochemical Pharmacology·Maxwell Afari Gyamfi, Yoko Aniya
Jun 1, 2002·Bioorganic & Medicinal Chemistry Letters·Danny BurgGerard J Mulder
Nov 21, 2002·IUBMB Life·Rachel I M van HaaftenAalt Bast
Jan 24, 2003·Expert Opinion on Therapeutic Targets·Laurent Gaté, Kenneth D Tew

❮ Previous
Next ❯

Citations

Jun 24, 2014·Ticks and Tick-borne Diseases·Rasha A GuneidyRagaa R Hamed
Oct 20, 2015·Fish Physiology and Biochemistry·R R HamedS S Abdel-Ghany
Aug 30, 2005·Life Sciences·Etsuki ShinnoYoko Aniya
Jun 30, 2015·The American Journal of Chinese Medicine·Anqi WangYitao Wang
Jul 25, 2015·Archiv der Pharmazie·Mustafa Muhlis Alparslan, Özkan Danış
Oct 4, 2016·Pharmacognosy Research·Nurul Afifah Mohd SallehMohd Rohaimi Ab Halim
Oct 28, 2016·Expert Opinion on Therapeutic Patents·Anil KumarRuchika Mittal
Jan 1, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Mary GulumianVanessa Steenkamp
Oct 3, 2018·Advanced Pharmaceutical Bulletin·Velid Unsal
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Rasha A GuneidyShaimaa A Allam

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.