Inhibition of glycine oxidation by pyruvate, alpha-ketoglutarate, and branched-chain alpha-keto acids in rat liver mitochondria: presence of interaction between the glycine cleavage system and alpha-keto acid dehydrogenase complexes

Archives of Biochemistry and Biophysics
H KochiK Ono

Abstract

Pyruvate, alpha-ketoglutarate, and branched-chain alpha-keto acids which were transaminated products of valine, leucine, and isoleucine inhibited glycine decarboxylation by rat liver mitochondria. However, glycine synthesis (the reverse reaction of glycine decarboxylation) was stimulated by those alpha-keto acids with the concomitant decarboxylation of alpha-keto acid added in the absence of NADH. Both the decarboxylation and the synthesis of glycine by mitochondrial extract were affected similarly by alpha-ketoglutarate and branched-chain alpha-keto acids in the absence of pyridine nucleotide, but not by pyruvate. This failure of pyruvate to have an effect was due to the lack of pyruvate oxidation activity in the mitochondrial extract employed. It indicated that those alpha-keto acids exerted their effects by providing reducing equivalents to the glycine cleavage system, possibly through lipoamide dehydrogenase, a component shared by the glycine cleavage system and alpha-keto acid dehydrogenase complexes. On the decarboxylation of pyruvate, alpha-ketoglutarate, and branched-chain alpha-keto acids in intact mitochondria, those alpha-keto acids inhibited one another. In similar experiments with mitochondrial extract, decarboxyla...Continue Reading

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Citations

Jul 1, 1988·Somatic Cell and Molecular Genetics·G OtulakowskiH F Willard
Oct 15, 1990·Biochemical and Biophysical Research Communications·K OnoH Kochi
Feb 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Z GregusC D Klaassen
Oct 11, 2001·Molecular Genetics and Metabolism·D A Applegarth, J R Toone
Jan 20, 2006·The FEBS Journal·Christos Chinopoulos, Vera Adam-Vizi
Aug 1, 2015·Neurochemical Research·Gerald A Dienel, Nancy F Cruz
Nov 24, 2012·Aging Cell·Jeffrey A ButlerShane L Rea
Aug 1, 1987·Archives of Biochemistry and Biophysics·D J CarothersM S Patel

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