Inhibition of granzyme B activity blocks inflammation induced by lipopolysaccharide through regulation of endoplasmic reticulum stress signaling in NK92 cells

Molecular Medicine Reports
Lei WangJing Tong

Abstract

Granzyme B (GrB) is a serine protease that is expressed in the lytic granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTL), and which has been widely reported to serve a crucial role for target cell apoptosis. GrB may serve a non‑cytotoxic role in inflammation, but the evidence remains unclear. The present study aimed to establish an inflammatory cell model by using NK92 cells stimulated with lipopolysaccharide (LPS) to investigate whether GrB was involved in the development of inflammation. The extracellular levels of tumor necrosis factor‑α (TNF‑α), interleukin‑1β (IL‑1β) and GrB were examined by ELISA, and it was demonstrated that LPS treatment increased the extracellular levels of TNF‑α, IL‑1β and GrB, and these increased expression levels were inhibited by pretreatment with the GrB inhibitor serpin A3N (SA3N). The protein expression levels of glucose‑regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), nuclear factor‑κB (NF‑κB), inhibitor of NF‑κB (IκBα) and GrB were examined by western blot analysis. The results demonstrated that LPS stimulation increased the expression levels of GRP78, CHOP, NF‑κB and GrB, and decreased the expression of IκBα, and these changes were inhibited by SA3N, which in...Continue Reading

References

Dec 15, 2000·The Journal of Biological Chemistry·J B AlimontiA H Greenberg
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A ThomasT J Ley
May 2, 2002·Cell·Sankar Ghosh, Michael Karin
May 20, 2003·Immunological Reviews·Sarah J LordR Chris Bleackley
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Amy S Lee
Apr 11, 2008·The Journal of Investigative Dermatology·Yuan-Ping HanWarren L Garner
Oct 28, 2008·Immunity·Sunil S MetkarChristopher J Froelich
Feb 17, 2009·Trends in Immunology·Christopher J FroelichMarkus M Simon
Jun 12, 2010·Immunological Reviews·Inna S AfoninaSeamus J Martin
Nov 3, 2010·International Journal of Oncology·Ilona Rousalova, Evzen Krepela
Dec 7, 2011·Critical Reviews in Immunology·Manuela RossolSunna Hauschildt
Jan 27, 2015·Clinical and Experimental Immunology·M J AshbrookN B Blatt
Sep 5, 2015·Journal of Neuroinflammation·Yohannes HaileFabrizio Giuliani
Jan 9, 2016·Human Molecular Genetics·Andoria TjondrokoesoemoJeffery D Molkentin

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