PMID: 9165534May 1, 1997Paper

Inhibition of growth of Dictyostelium discoideum amoebae by bisphosphonate drugs is dependent on cellular uptake

Pharmaceutical Research
M J RogersD J Watts

Abstract

The aim of the study was to determine whether bisphosphonates are internalised by Dictyostelium amoebae and whether cellular uptake is required for their growth-inhibitory effects. Bisphosphonates inhibit growth of amoebae of the slime mould Dictyostelium discoideum, by mechanisms that appear to be similar to those that cause inhibition of osteoclastic bone resorption. Cell-free extracts prepared from amoebae that had been incubated with bisphosphonates were analysed by 31P-n.m.r, spectroscopy or ion-exchange f.p.l.c., to identify the presence of bisphosphonates or bisphosphonate metabolites respectively. The growth-inhibitory effect of bisphosphonates towards Dictyostelium amoebae was also examined under conditions in which pinocytosis was inhibited. All of the bisphosphonates studied were internalised by Dictyostelium amoebae, probably by fluid-phase pinocytosis, and could be detected in cell-free extracts. Amoebae that were prevented from internalising bisphosphonates by pinocytosis were markedly resistant to the growth-inhibitory effects of these compounds. In addition, bisphosphonates encapsulated within liposomes were more potent growth inhibitors of Dictyostelium owing to enhanced intracellular delivery of bisphosphonate...Continue Reading

Citations

May 23, 1998·Annual Review of Pharmacology and Toxicology·G A Rodan
Feb 12, 1999·Pharmacology & Toxicology·S LuurilaR Ylitalo
Oct 6, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R G RussellH A Fleisch
May 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J E GroveD J Watts
Oct 13, 2005·Lupus·M BevilacquaM Barbagallo
Nov 7, 2008·Clinical Journal of the American Society of Nephrology : CJASN·Nigel D ToussaintPeter G Kerr
Nov 15, 1997·Cancer·M J RogersR G Russell
Oct 12, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J LiH Norimatsu
Jun 20, 2002·Pharmacology & Toxicology·Ritva YlitaloPauli Ylitalo
Feb 6, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Julie C Frith, Michael J Rogers
Jul 13, 2006·Annals of the New York Academy of Sciences·R Graham G Russell
Aug 13, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oh SasakiMakoto Dohi
Feb 7, 2014·TheScientificWorldJournal·Wen-Chih LiuKuo-Cheng Lu
Feb 28, 2017·Journal for Immunotherapy of Cancer·Mohanad H NadaCraig T Morita
May 16, 2014·Oral Diseases·K C OhJ-H Kim
Mar 30, 2002·General Pharmacology·R Ylitalo
Jan 6, 2000·Archives of Biochemistry and Biophysics·J D BergstromG Rodan
Feb 14, 2002·International Journal for Parasitology·Michael T McIntosh, Akhil B Vaidya
May 13, 1999·Bone·M J RogersR G Russell
Jul 10, 2003·Biochemical and Biophysical Research Communications·Dorothea Strobach, Reinhard L Lorenz
Aug 8, 2021·International Journal of Molecular Sciences·Liberato GiardulloFrancesco Paolo Cantatore
Oct 2, 2007·Bioorganic & Medicinal Chemistry·Andrew J WiemerDavid F Wiemer
Mar 30, 2010·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Nigel D ToussaintPeter G Kerr
May 11, 2011·Bone·R Graham G Russell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.