Inhibition of hair follicle growth by a laminin-1 G-domain peptide, RKRLQVQLSIRT, in an organ culture of isolated vibrissa rudiment

The Journal of Investigative Dermatology
Kazuhiro HayashiY Kadoya

Abstract

We established a serum-free organ culture system of isolated single vibrissa rudiments taken from embryonic day 13 mice. This system allowed us to test more than 30 laminin-derived cell adhesive peptides to determine their roles on the growth and differentiation of vibrissa hair follicles. We found that the RKRLQVQLSIRT sequence (designated AG-73), which mapped to the LG-4 module of the laminin-alpha1 chain carboxyl-terminal G domain, perturbed the growth of hair follicles in vitro. AG-73 is one of the cell-binding peptides identified from more than 600 systematically synthesized 12 amino acid peptides covering the whole amino acid sequence of the laminin-alpha1, -beta1, and -gamma1 chains, by cell adhesion assay. Other cell-adhesive laminin peptides and a control scrambled peptide, LQQRRSVLRTKI, however, failed to show any significant effects on the growth of hair follicles. The AG-73 peptide binds to syndecan-1, a transmembrane heparan-sulfate proteoglycan. Syndecan-1 was expressed in both the mesenchymal condensation and the epithelial hair peg of developing vibrissa, suggesting that AG-73 binding to the cell surface syndecan-1 perturbed the epithelial-mesenchymal interactions of developing vibrissa. The formation of hair bu...Continue Reading

References

Feb 1, 1992·Trends in Genetics : TIG·M H Hardy
Jan 1, 1991·Anatomy and Embryology·Y Kadoya, S Yamashina
Dec 1, 1995·The Journal of Biological Chemistry·J H MinerJ R Sanes
Feb 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·S M BernierY Yamada
Mar 1, 1994·The Journal of Cell Biology·M L Matter, G W Laurie
Mar 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Y ZhangP Ekblom
Dec 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·J Liu, R Mayne
Aug 15, 1997·Microscopy Research and Technique·R B WidelitzC M Chuong
Jul 22, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y KadoyaY Yamada
Jul 29, 1998·International Journal of Cancer. Journal International Du Cancer·W H KimY Yamada
Nov 26, 1998·The Journal of Biological Chemistry·M NomizuY Yamada
Jan 12, 1999·Developmental Biology·C ChiangA A Dlugosz
Apr 3, 1999·Circulation Research·M L PonceK M Malinda
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KishimotoR E Burgeson
Nov 13, 1999·Nature Cell Biology·V A BotchkarevR Paus
Dec 3, 1999·The British Journal of Dermatology·M AkiyamaT Nishikawa
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Jun 22, 2000·Archives of Biochemistry and Biophysics·M NomizuY Yamada
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.