Inhibition of heat-induced denaturation of albumin by nonsteroidal antiinflammatory drugs (NSAIDs): pharmacological implications

Archives of Pharmacal Research
L SasoB Silvestrini

Abstract

The activity of nonsteroidal antiinflammatory drugs (NSAIDs) in rheumatoid arthritis is not only due to the inhibition of the production of prostaglandins, which can even have beneficial immunosuppressive effects in chronic inflammatory processes. Since we speculated that these drugs could also act by protecting endogenous proteins against denaturation, we evaluated their effect on heat-induced denaturation human serum albumin (HSA) in comparison with several fatty acids which are known to be potent stabilizers of this protein. By the Mizushimas assay and a recently developed HPLC assay, we observed that NSAIDs were slightly less active [EC50 to approximately 10(-5)-10(-4) M] than FA and that the HPLC method was less sensitive but more selective than the turbidimetric assay, i.e. it was capable of distinguishing true antiaggregant agents like FA and NSAIDs from substances capable of inhibiting the precipitation of denatured protein aggregates. In conclusion, this survey could be useful for the development of more effective agents in protein condensation diseases like rheumatic disorders, cataract and Alzheimers disease.

References

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Citations

Oct 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·C S Chidan KumarHoong-Kun Fun
Sep 4, 2014·Journal of Evidence-based Complementary & Alternative Medicine·Murad Ali KhanSamreen Pervez
Dec 21, 2016·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Amadou DawéBonabenture Tchaleu Ngadjui
Dec 22, 2020·Frontiers in Pharmacology·Antonella SmeriglioDomenico Trombetta
Mar 15, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Wróbel-Biedrawa DagmaraPodolak Irma
Jul 30, 2019·Journal of Photochemistry and Photobiology. B, Biology·Sekar VijayakumarBaskaralingam Vaseeharan
Oct 27, 2004·Experimental Eye Research·Martin IwigHans Gert Struck

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