Inhibition of Heat Shock proteins HSP90 and HSP70 induce oxidative stress, suppressing cotton fiber development

Scientific Reports
Anshulika SableSamir V Sawant

Abstract

Cotton fiber is a specialized unicellular structure useful for the study of cellular differentiation and development. Heat shock proteins (HSPs) have been shown to be involved in various developmental processes. Microarray data analysis of five Gossypium hirsutum genotypes revealed high transcript levels of GhHSP90 and GhHSP70 genes at different stages of fiber development, indicating their importance in the process. Further, we identified 26 and 55 members of HSP90 and HSP70 gene families in G. hirsutum. The treatment of specific inhibitors novobiocin (Nov; HSP90) and pifithrin/2-phenylethynesulfonamide (Pif; HSP70) in in-vitro cultured ovules resulted in a fewer number of fiber initials and retardation in fiber elongation. The molecular chaperone assay using bacterially expressed recombinant GhHSP90-7 and GhHSP70-8 proteins further confirmed the specificity of inhibitors. HSP inhibition disturbs the H2O2 balance that leads to the generation of oxidative stress, which consequently results in autophagy in the epidermal layer of the cotton ovule. Transmission electron microscopy (TEM) of inhibitor-treated ovule also corroborates autophagosome formation along with disrupted mitochondrial cristae. The perturbations in transcript p...Continue Reading

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Citations

Jun 29, 2018·The Plant Journal : for Cell and Molecular Biology·Verandra KumarSamir V Sawant
Nov 13, 2018·Journal of the Science of Food and Agriculture·Yuan SuiXianghong Meng
Nov 7, 2019·Journal of Experimental Botany·Nathan M RowarthArunika Hlan Gunawardena
Jan 30, 2020·Plant, Cell & Environment·Marine SauxChristophe Bailly
Oct 14, 2020·Plant, Cell & Environment·Farhad Masoomi-AladizgehBrian J Atwell
Feb 21, 2021·Molecular Biology Reports·Muhammad Asif SaleemZia Ullah Zia
Jul 18, 2020·International Journal of Biological Macromolecules·Lichen WangMuhammad Mujtaba
Jul 27, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fereshteh RadmaneshAli Shalizar-Jalali
Sep 2, 2021·Journal of Neurophysiology·Fengfang WuJun Liao
Nov 28, 2021·The Plant Journal : for Cell and Molecular Biology·Farhad Masoomi-AladizgehBrian J Atwell

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Datasets Mentioned

BETA
AY375335
PRJNA397595

Methods Mentioned

BETA
protein folding
scanning electron microscopy
RNAseq

Software Mentioned

ClustalW
MeV
Mapman
ABI7500 SDS
RNAseq
MEGA
Tophat
FASTX
CELLOv
DNASTAR QSeq

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