Inhibition of heparan sulfate and chondroitin sulfate proteoglycan biosynthesis.

The Journal of Biological Chemistry
Dinesh R GarudBalagurunathan Kuberan

Abstract

Proteoglycans (PGs) are composed of a protein moiety and a complex glycosaminoglycan (GAG) polysaccharide moiety. GAG chains are responsible for various biological activities. GAG chains are covalently attached to serine residues of the core protein. The first step in PG biosynthesis is xylosylation of certain serine residues of the core protein. A specific linker tetrasaccharide is then assembled and serves as an acceptor for elongation of GAG chains. If the production of endogenous GAG chains is selectively inhibited, one could determine the role of these endogenous molecules in physiological and developmental functions in a spatiotemporal manner. Biosynthesis of PGs is often blocked with the aid of nonspecific agents such as chlorate, a bleaching agent, and brefeldin A, a fungal metabolite, to elucidate the biological roles of GAG chains. Unfortunately, these agents are highly lethal to model organisms. Xylosides are known to prime GAG chains. Therefore, we hypothesized that modified xylose analogs may able to inhibit the biosynthesis of PGs. To test this, we synthesized a library of novel 4-deoxy-4-fluoroxylosides with various aglycones using click chemistry and examined each for its ability to inhibit heparan sulfate and c...Continue Reading

References

Jul 15, 1988·Biochemical and Biophysical Research Communications·D E Humphries, J E Silbert
Sep 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M SalmivirtaU Lindahl
Dec 14, 1999·The Journal of Biological Chemistry·F SafaiyanM Salmivirta
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Sep 1, 2000·Carbohydrate Research·K DaxB J Paul
Dec 5, 2000·Current Opinion in Chemical Biology·R Sasisekharan, G Venkataraman
Jun 5, 2002·Annual Review of Biochemistry·Jeffrey D Esko, Scott B Selleck
Oct 30, 2004·Glycoconjugate Journal·Nancy B Schwartz, Miriam Domowicz
Dec 26, 2006·The Journal of Biological Chemistry·Karen CuellarMyron E Hinsdale
Jan 20, 2007·Nature Chemical Biology·Jeremy E Turnbull, Robert A Field
Dec 19, 2007·Chembiochem : a European Journal of Chemical Biology·Balagurunathan KuberanAnh Do

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Citations

Dec 15, 2012·Pharmaceutical Research·Mamta Kapoor, Diane J Burgess
Dec 23, 2011·Biochemistry Research International·Alexander V Maksimenko, Askar D Turashev
Oct 23, 2013·Angiogenesis·Xander M R van Wijk, Toin H van Kuppevelt
Nov 19, 2015·Carbohydrate Research·Karin ThorsheimUlf Ellervik
Nov 26, 2010·Biochemical and Biophysical Research Communications·Karthik RamanBalagurunathan Kuberan
Apr 2, 2015·Biochimica Et Biophysica Acta·Achilleas D TheocharisNikos K Karamanos
Apr 26, 2013·ACS Chemical Biology·Thao K N NguyenBalagurunathan Kuberan
Dec 29, 2016·Medicinal Research Reviews·Giancarlo Ghiselli
Dec 25, 2019·The Journal of Cell Biology·Alexander Buffone, Valerie M Weaver
Feb 19, 2020·Journal of Cellular and Molecular Medicine·Ying LanLijuan Zhang
Jun 1, 2013·Biomolecular Concepts·Vimal P SwarupBalagurunathan Kuberan
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Maanasa RavikumarSimon M Cool
Jul 3, 2021·International Journal of Molecular Sciences·Marissa L Maciej-HulmeCatherine L R Merry
Oct 24, 2017·Accounts of Chemical Research·Jie Shi Chua, Balagurunathan Kuberan
Dec 15, 2020·Molecular Pharmaceutics·Mausam KalitaDavid M Wilson
Nov 16, 2010·Bioorganic & Medicinal Chemistry Letters·Yasuhiro TsuzukiMamoru Koketsu
May 31, 2011·Bioorganic & Medicinal Chemistry·Anna SiegbahnUlf Ellervik

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