Inhibition of hepatic aryl hydrocarbon hydroxylase by 3-methylcholanthrene, 7,8-benzoflavone and other inducers added in vitro.

Chemico-biological Interactions
P H JellinckA M Newcombe

Abstract

A close correlation has been observed between the ability of aromatic polycyclic hydrocarbons and 7,8-benzoflavone (7,8-BF) to induce hepatic aryl hydrocarbon hydroxylase (AHH) in vivo and to inhibit the induced enzyme system in vitro. The activity of this mono-oxygenase was measured by the conversion of 14C-labeled dimethylbenz(a)anthracene (DMBA) or benzo(a)pyrene (BP) to water-soluble products by rat liver preparations (8000 X g supernatant). DMBA as substrate had the advantage over BP in giving a wider range of ethyl acetate-soluble metabolites and allowing the observation of changes in the pattern of these products following injection or addition of the inducing agents. This property was used to detect low concentration (0.1 muM) of polycyclic hydrocarbons which are strong AHH inducers and which may also be carcinogenic. The liver preparation was active for several months when stored at --20 degrees. A possible mechanism of action for the in vitro behaviour of polycyclic hydrocarbons and 7,8-BF towards AHH is proposed.

References

Jan 1, 1972·Annual Review of Pharmacology·J R GilletteH A Sasame
Dec 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·H V GelboinL Sachs
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·N Kinoshita, H V Gelboin
Apr 28, 1973·Biochimica Et Biophysica Acta·P H Jellinck, G Smith
Mar 27, 1968·Biochemical and Biophysical Research Communications·A HildebrandtR W Estabrook
Sep 8, 1966·Biochemical and Biophysical Research Communications·N E Sladek, G J Mannering
Dec 1, 1967·Biochemical Pharmacology·P H JellinckM Garland
Oct 15, 1971·FEBS Letters·P L. GroverP Sims
Apr 1, 1965·The Journal of Endocrinology·P H JELLINCK, I LUCIEER
Nov 30, 1967·Biochemical and Biophysical Research Communications·A P AlvaresR Kuntzman

❮ Previous
Next ❯

Citations

Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·P H JellinckH L Bradlow
Jul 16, 1980·Biochemical and Biophysical Research Communications·J J Stegeman, B R Woodin
Jun 1, 1980·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·W L AlworthT Viswanathan
Jan 1, 1981·Drug Metabolism Reviews·B Testa, P Jenner
Oct 1, 1986·European Journal of Drug Metabolism and Pharmacokinetics·P Kourounakis, E Rekka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.