PMID: 9181525May 1, 1997Paper

Inhibition of hepatitis B virus by retroviral vectors expressing antisense RNA

Journal of Viral Hepatitis
W Ji, C W Si

Abstract

Two retroviral vectors carrying an antisense gene from the hepatitis B virus (HBV) preS/S or preC/C were constructed and used to infect the human hepatoblastoma cell line 2.2.15, which expresses HBV surface antigen (HBsAg), HBV e antigen (HBeAg) and releases HBV particles. The results showed that the inhibitory effects of antisense gene transfer, mediated by retroviral vectors on the expression of HBV antigens, appeared as early as day 3 after transduction, reached a maximum on day 5 and persisted for at least 11 days. Our data indicate that, on day 5 after introduction, antisense preS/S inhibited HBsAg and HBeAg expression by 71% and 23%, and the antisense preC/C inhibited HBsAg and HBeAg expression by 23% and 59%. HBV DNA production, in the supernatant of the 2.2.15 cells transduced with either antisense preS/S or preC/C, was also reduced on day 5, but the viability of the 2.2.15 cells was not affected. Our results demonstrate that the replication and expression of HBV can be inhibited through antisense gene transfer mediated by retroviral vectors and that the antisense-preC/C or antisense-preS/S may be potentially useful for clinical gene therapy against HBV.

Citations

Jun 22, 2000·Journal of Medical Virology·C G AnastassopoulouA Hatzakis
Sep 20, 2002·Antisense & Nucleic Acid Drug Development·Theresa May Chin TanSeng Gee Lim
Jun 25, 2002·Journal of Clinical Gastroenterology·Richard A Willson
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Aug 21, 2004·Journal of Clinical Gastroenterology·Richard A Willson
Jul 5, 2005·Expert Opinion on Investigational Drugs·J Torresi, S A Locarnini
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