Inhibition of hepatitis B Virus replication by hepatocyte nuclear factor 4-alpha specific short hairpin RNA

Liver International : Official Journal of the International Association for the Study of the Liver
F HeHong Tang

Abstract

Previous studies showed that hepatocyte nuclear factor 4α (HNF4α) may play a critical role in hepatitis B virus (HBV) replication. This study aimed to investigate the effect of knocking down of HNF4α with RNA interference technique on HBV replication in a HBV replication mouse model. Four HNF4α, specific short hairpin RNA (shRNA)-producing plasmids were constructed. HBV mRNA and DNA replication intermediates were analysed using Northern and Southern blot respectively. The expression of HNF4α and HBV core antigen (HBcAg) was detected using immunohistochemistry technique. One of the HNF4α shRNAs, HNF4α shRNA1, efficiently inhibited the expression of HNF4α in HepG2 cells and mice liver. HBV RNA transcripts and DNA replication intermediates in HNF4α shRNA1 group were decreased 67.3 and 76%, respectively, in HepG2 cells, and 68.1 and 70.6% in mice liver respectively. The expression level of HBcAg in the liver was also decreased with the inhibition of HNF4α expression. These results suggested that decreasing of HNF4α expression was associated with the reduced level of HBV replication in HepG2 cells and mice liver. These data indicated that HNF4α played a critical role in HBV replication in vivo, and HNF4α shRNA could inhibit HBV repl...Continue Reading

References

Jan 16, 1991·Journal of the National Cancer Institute·P VerrelleJ Chassagne
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·H Tang, A McLachlan
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Jul 24, 2002·The American Journal of Gastroenterology·George V PapatheodoridisVasilios Papadimitropoulos
Oct 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Priscilla L YangFrancis V Chisari
Mar 12, 2004·The New England Journal of Medicine·Don Ganem, Alfred M Prince
Oct 22, 2004·The New England Journal of Medicine·Mario Stevenson
Aug 17, 2005·Seminars in Liver Disease·Brian J McMahon
Dec 13, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sergio CarmonaPatrick Arbuthnot
Dec 17, 2005·Journal of Hepatology·Antonio CraxìCalogero Cammà
Mar 15, 2006·Gastroenterology·Uchenna H IloejeUNKNOWN Risk Evaluation of Viral Load Elevation and Associated Liver Disease/Cancer-In HBV (the REVEAL-HBV) Study Group
May 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nguyen L ToanC-Thomas Bock
Jul 5, 2006·The American Journal of Gastroenterology·Gang ChenAlison A Evans
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Roger Williams
Jan 11, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Marc S WeinbergPatrick Arbuthnot
Jan 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Vesna S StanulovićWouter H Lamers
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wei-Hong WenAn-Gang Yang
Sep 20, 2007·World Journal of Gastroenterology : WJG·Feng-Jun LiuHong Tang
Dec 13, 2007·Pharmaceutical Research·Yong ChenRam I Mahato
Jan 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Henry Lik-Yuen ChanTony Shu-Kam Mok
Sep 23, 2008·Cell·Joel R Neilson, Phillip A Sharp

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Citations

Oct 5, 2013·Expert Opinion on Drug Delivery·Roberto MolinaroMassimo Fresta
Sep 4, 2014·BioMed Research International·Josune TorrecillaAna del Pozo-Rodríguez
Jul 14, 2019·Journal of Medical Virology·Shu FengChuanmin Tao
Sep 5, 2019·International Journal of Molecular Sciences·Nur K Mohd-IsmailYee-Joo Tan
Feb 6, 2020·Viruses·Kristi L TurtonTrushar R Patel
Sep 10, 2016·World Journal of Gastroenterology : WJG·Doo Hyun KimKyun-Hwan Kim
Sep 19, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yanchao PanJing Yuan
Aug 23, 2019·World Journal of Gastroenterology : WJG·Matthew M YehSayed S Daoud

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