Inhibition of Hepatitis B virus replication by phospholipid scramblase 1 in vitro and in vivo

Antiviral Research
Jing YangShengqi Wang

Abstract

Human Phospholipid scramblase 1 (PLSCR1) is an α/β interferon-inducible protein that mediates antiviral activity against RNA viruses including vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV). In the present study, we investigated the antiviral activity of PLSCR1 protein against HBV (Hepatitis B virus). Firstly, PLSCR1 mRNA and protein expression was found to be downregulated in HepG2 cells after HBV infection. Then by performing co-transient-transfection experiments in cells and hydrodynamics-based transfection experiments in mice using a HBV expression plasmid and a PLSCR1 expression plasmid, we found that PLSCR1 inhibited HBV replication in vitro and in vivo through a significant reduction in the synthesis of viral proteins, DNA replicative intermediates and HBV RNAs. We also demonstrated that the antiviral action of PLSCR1 against HBV occurs, partly at least, by activating the Jak/Stat pathway. In conclusion, our results suggest that the expression of PLSCR1 is involved in HBV replication and that PLSCR1 has antiviral activity against HBV.

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Citations

Dec 6, 2014·FEBS Letters·Karthik M KodigepalliMeera Nanjundan
Mar 19, 2013·Biochemical and Biophysical Research Communications·Shuichi Kusano, Yoshito Eizuru
May 22, 2016·BMC Biochemistry·Ulaganathan SivagnanamSathyanarayana N Gummadi
Mar 11, 2017·PloS One·Asma Kassas-GuediriMarc Benhamou
Sep 2, 2017·Vaccines·Ana Maria Ortega-Prieto, Marcus Dorner
Jun 1, 2019·AIDS·Ana C PaimAndrew D Badley
Aug 31, 2018·Frontiers in Immunology·Ming-Hsun Tsai, Chien-Kuo Lee
May 24, 2019·Systems Medicine : Journal of Medical Systems Biology and Network Medicine·Christian WiwieJan Baumbach
Apr 4, 2021·Viruses·Joe McKellarCaroline Goujon

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