Inhibition of hippocampal estrogen synthesis causes region-specific downregulation of synaptic protein expression in hippocampal neurons

Hippocampus
J Prange-KielGabriele M Rune

Abstract

Previous studies have shown that synapses and expression of synaptic proteins in hippocampal neurons are regulated by hippocampus-derived estradiol. Here, we compared the effects of this paracrine regulation in different hippocampal regions. In tissue sections, immunohistochemistry followed by semiquantitative image analysis revealed a three-fold higher expression of steroidogenic acute regulatory protein (StAR) and aromatase in neurons of the CA3 than that of the CA1 region and in granule cells. Next, we treated hippocampal cell cultures with letrozole, an aromatase inhibitor, which resulted in a dose-dependent decrease in the release of 17beta-estradiol into the medium and in a dose-dependent downregulation of spinophilin and synaptophysin expression in dissociated hippocampal neurons. The downregulation of synaptic protein expression was restored by simultaneous application of letrozole together with estradiol. In response to a defined dose of letrozole, the downregulation of spinophilin expression was significantly stronger in CA1 neurons and in granule cells, than in cells of the CA3 region in slice cultures. With synaptophysin, downregulation was stronger in stratum lucidum of CA3 than in stratum radiatum of CA1. Both reg...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Feb 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jürgen GeislerPer Eystein Lønning
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·John R PuddefootGavin P Vinson
Mar 18, 2003·Pituitary·José CarreteroRicardo Vázquez
Apr 18, 2003·Hippocampus·Janine Prange-KielGabriele M Rune
Dec 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yasushi HojoSuguru Kawato
Jul 2, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Oliver KretzGabriele M Rune

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Citations

Apr 22, 2011·Cell and Tissue Research·Gianluca LeporeVittorio Farina
May 9, 2012·Reviews in Endocrine & Metabolic Disorders·Joseph Nuñez
Jul 27, 2011·Neuroscience Bulletin·Fan-Tao MengJiang-Ning Zhou
Aug 6, 2014·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·N BrandtG M Rune
Jun 1, 2014·Frontiers in Neuroendocrinology·Ashley M Fortress, Karyn M Frick
Jan 30, 2008·The Journal of Cell Biology·Janine Prange-KielGabriele M Rune
Aug 3, 2013·Progress in Histochemistry and Cytochemistry·Janine Prange-KielGabriele M Rune
Feb 10, 2010·Journal of Neuropathology and Experimental Neurology·Kai-Liang WuC Shun Wong
Dec 6, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Lars FesterGabriele M Rune
Nov 15, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Yuuki OoishiHideo Mukai
Nov 17, 2009·Biochimica Et Biophysica Acta·Hideo MukaiMari Ogiue-Ikeda
Jun 30, 2009·Journal of Chemical Neuroanatomy·Stefano GottiGiancarlo Panzica
May 20, 2009·Frontiers in Neuroendocrinology·Colin J SaldanhaBradley J Walters
May 21, 2015·Hormones and Behavior·Maya Frankfurt, Victoria Luine
May 12, 2015·Hormones and Behavior·Karyn M Frick
Apr 12, 2008·Brain Research·Josue G YagueJohn H Morrison
Feb 29, 2008·Hippocampus·Fenghua ChenJens R Nyengaard
Oct 10, 2006·Journal of Neuroendocrinology·C von SchassenG M Rune
Jul 8, 2008·Journal of Neuroendocrinology·L M Garcia-Segura
Oct 18, 2006·Journal of Neuroscience Research·Takamitsu IkedaMaki K Yamada
Oct 10, 2009·Brain Research·Josue G YagueAlberto Muñoz
Dec 3, 2014·Brain Research·Lars Fester, Gabriele M Rune
Jan 1, 2010·Hormone Molecular Biology and Clinical Investigation·Iñigo AzcoitiaLuis M Garcia-Segura
Oct 1, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Lars FesterGabriele M Rune
Oct 30, 2016·The Journal of Pharmacology and Experimental Therapeutics·Amanda Crider, Anilkumar Pillai
Nov 20, 2016·Journal of Neurochemistry·Lars FesterGabriele M Rune
Nov 28, 2017·BMC Complementary and Alternative Medicine·Deok-Sang HwangMyung Sook Oh
Oct 1, 2013·Pharmacological Reviews·Deepak P SrivastavaPeter Penzes

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