Inhibition of HIV-1 replication by a Tat transdominant negative mutant in human peripheral blood lymphocytes from healthy donors and HIV-1-infected patients

Gene Therapy
C RossiA Caputo

Abstract

It was previously shown that a tat mutant (tat22) where cysteine 22 is substituted by glycine behaves as a transdominant negative mutant in Jurkat T cells lytically or latently infected by HIV-1. In this study we demonstrate that tat22 controls HIV-1 replication in primary cells. This effect was observed both after in vitro infection of peripheral blood mononuclear cells (PBMCs) from normal donors and after reactivation of the latent infection in PBMCs from seropositive patients. The antiviral effect of tat22 was limited to conditions of low virus production. The use of tat22 may be promising for a gene therapy approach to AIDS during the asymptomatic phase of the disease allowing control of virus replication in infected cells and inhibition of virus spread to uninfected cells.

Citations

Feb 13, 2002·AIDS Patient Care and STDs·Mario R Mautino, Richard A Morgan
Sep 8, 2009·Oligonucleotides·Thea Müller-KullerStefan A Klein
Nov 11, 2009·PloS One·Luke W MeredithDavid Harrich
Aug 29, 2003·Expert Opinion on Biological Therapy·Ananthalakshmi PoluriRichard E Sutton
Aug 18, 2012·Advances in Virology·Luna LiBrian Wigdahl
Nov 18, 2005·Advances in Genetics·Mary K Lewinski, Frederic D Bushman
Oct 24, 2013·Reviews in Medical Virology·Cecilia T Costiniuk, Mohammad-Ali Jenabian
Jan 6, 2021·International Journal of Molecular Sciences·Aurelio CafaroBarbara Ensoli

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