Inhibition of HIV-1 replication by combined expression of gag dominant negative mutant and a human ribonuclease in a tightly controlled HIV-1 inducible vector

Gene Therapy
A CaraG L Gusella

Abstract

An HIV-1-based expression vector has been constructed that produces protective genes tightly regulated by HIV-1 Tat and Rev proteins. The vector contains either a single protective gene (HIV-1 gag dominant negative mutant (delta-gag)) or a combination of two different protective genes (delta-gag and eosinophil-derived neurotoxin (EDN), a human ribonuclease) which are expressed from a dicistronic mRNA. After stable transfection of CEM T cells and following challenge with HIV-1, viral production was completely inhibited in cells transduced with the vector producing both delta-gag and EDN and delayed in cells producing delta-gag alone. In addition, cotransfection of HeLa-Tat cells with an infectious HIV-1 molecular clone and either protective vector demonstrated that the HIV-1 packaging signals present in the constructs were functional and allowed the efficient assembly of the protective RNAs into HIV-1 virions, thus potentially transmitting protection to the HIV-1 target cells.

Citations

Feb 13, 2002·AIDS Patient Care and STDs·Mario R Mautino, Richard A Morgan
Dec 5, 1998·Journal of the National Cancer Institute·D L Newton, S M Rybak
Jan 14, 2012·Critical Reviews in Microbiology·Anu Malik, Janendra K Batra
Nov 13, 2014·Expert Opinion on Therapeutic Targets·Elena Herrera-Carrillo, Ben Berkhout
Dec 10, 1999·Experimental Cell Research·S M Rybak, D L Newton
Jul 21, 2015·Viruses·Elena Herrera-Carrillo, Ben Berkhout
Jan 4, 2006·The Journal of Gene Medicine·Elena FedorovaG Luca Gusella
Apr 28, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·John H ChidlowChristopher G Kevil
Jun 15, 2007·American Journal of Physiology. Cell Physiology·Shuji KondoChristine M Sorenson
Feb 27, 2010·Nucleic Acids Research·Bo ChenFenfei Leng
Mar 1, 2017·Biotechnology and Bioengineering·Cheryl M ImmethunTae Seok Moon
Mar 11, 2020·Molecular Therapy. Methods & Clinical Development·Alessandra GallinaroAndrea Cara

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