PMID: 7539076Jan 1, 1994Paper

Inhibition of HIV-1 reverse transcriptase by defined template/primer DNA oligonucleotides: effect of template length and binding characteristics

Journal of Enzyme Inhibition
H Idriss, D K Stammers

Abstract

The interaction of partially double stranded DNA oligonucleotides with HIV-1 RT was studied by investigating their ability to inhibit the homopolymeric poly(rC) directed (dG) synthesis reaction. A 20/18mer oligonucleotide, with a sequence based on the Lys3-tRNA primer region, showed stronger inhibition of the homopolymeric RT reaction than a G/C rich oligonucleotide series lacking or possessing a hairpin moiety. Interaction of the enzyme with the G/C rich oligonucleotides, as determined by IC50 measurements, was insensitive to the extent of the unpaired template region at the 3' or 5' position. Addition of a hairpin moiety, composed of four thymidine bases, onto G/C rich oligonucleotides increase their inhibitory potency (at least six times) and shifted the mode of inhibition of RT to competitive with respect to poly (rC).(dG), which was otherwise mixed (competitive/noncompetitive) for the linear G/C rich and 20/18mer oligonucleotides. The results indicate that interaction of the enzyme with the primer/template stem, but not with the unpaired template region, is an important step in complex formation.

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A B WestR D Kolodner
Sep 28, 1990·Science·H MitsuyaS Broder
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z HostomskyB R Nodes
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P A FurmanS Hopkins
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M E GoldmanJ M Hoffman
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D L RomeroL Resnick
May 26, 1987·Nucleic Acids Research·T R WebbP G Ng
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M MatsukuraS Broder
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Jacobo-MolinaP Clark
Aug 25, 1993·Nucleic Acids Research·M KruhøfferF Grosse

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