PMID: 7537875Apr 11, 1995Paper

Inhibition of HIV-1 reverse transcription by triple-helix forming oligonucleotides with viral RNA

Nucleic Acids Research
S VolkmannK Moelling

Abstract

Reverse transcription of retroviral RNA into double-stranded DNA is catalyzed by reverse transcriptase (RT). A highly conserved polypurine tract (PPT) on the viral RNA serves as primer for plus-strand DNA synthesis and is a possible target for triple-helix formation. Triple-helix formation during reverse transcription involves either single-stranded RNA or an RNA.DNA hybrid. The effect of triple-helix formation on reverse transcription has been analyzed here in vitro using a three-strand-system consisting of an RNA.DNA hybrid and triplex-forming oligonucleotides (TFOs) consisting either of DNA or RNA. Three strand triple-helices inhibit RNase H cleavage of the PPT-RNA.DNA hybrid and initiation of plus-strand DNA synthesis in vitro. Triple-helix formation on a single-stranded RNA target has also been tested in a two-strand-system with TFOs comprising Watson-Crick and Hoogsteen base-pairing sequences, both targeted to the PPT-RNA, on a single strand connected by a linker (T)4. TFOs prevent RNase H cleavage of the PPT-RNA and initiation of plus-strand DNA synthesis in vitro. In cell culture experiments one TFO is an efficient inhibitor of retrovirus replication, leading to a block of p24 synthesis and inhibition of syncytia format...Continue Reading

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Citations

Oct 1, 1996·Plant Molecular Biology·P KlaffG Steger
Feb 24, 2001·Nucleosides, Nucleotides & Nucleic Acids·T HiratouH Takaku
Aug 31, 2012·AIDS Research and Human Retroviruses·Felix BroeckerKarin Moelling
Feb 1, 1996·Nucleic Acids Research·H Knudsen, P E Nielsen
Jul 11, 2003·Nucleic Acids Research·Sergei IvanovMarina B Gottikh
Apr 29, 2009·Virology Journal·Alexander FalkenhagenKarin Moelling
Jun 2, 2006·FEBS Letters·Karin MoellingJochen Heinrich
Oct 3, 2006·Molecular Carcinogenesis·Qi WuKaren M Vasquez
Apr 28, 1997·Biochemical and Biophysical Research Communications·S TsukaharaH Takaku
Dec 8, 2005·Bioorganic & Medicinal Chemistry Letters·Aya ShibataYukio Kitade
May 20, 2008·Biochimie·Nicholas N Shaw, Dev P Arya
Feb 6, 2020·Chemical Communications : Chem Comm·Hisae Tateishi-Karimata, Naoki Sugimoto
Aug 16, 2001·The Journal of Biological Chemistry·K M VasquezP M Glazer
Feb 12, 1998·Biochemical and Molecular Medicine·Y LavrovskyA K Roy

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