PMID: 8955849Dec 1, 1996Paper

Inhibition of HIV replication by immunoliposomal antisense oligonucleotide

Antiviral Research
M P SelvamJ S Epstein

Abstract

The sequence-specific suppression of HIV-1 replication using CD4 monoclonal-antibody-targeted liposomes, containing Rev antisense phosphorothioate oligonucleotides is described. Liposomes were prepared by encapsulating the 20-mer antisense DNA sequence of the rev HIV-1 regulatory gene, in the form of a phosphorothioate oligonucleotide. Specific targeting was accomplished by conjugating anti-CD4 mouse monoclonal antibody to the surface of the liposomes. HIV-1-infected H9 cells as well as peripheral blood T-lymphocytes were incubated with the immunoliposomes of antisense found to have potential antiviral effect. HIV-1 replication was reduced by 85% in antisense immunoliposome-treated H9 cells and peripheral blood lymphocytes, whereas the inhibition of HIV-1 replication was not observed using either empty immunoliposomes or immunoliposomes containing scrambled Rev phosphorothioate oligonucleotide sequences. The antiviral activity of both the free and the encapsulated oligonucleotides were assessed by p24, reverse transcriptase (RT) assays and polymerase chain reaction (PCR) analysis. Liposome preparations demonstrated minimal toxicity in H9 as well as in peripheral blood lymphocyte cell culture experiments. These in vitro culture ...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J LisziewiczR Gallo
Feb 25, 1991·Nucleic Acids Research·J P ShawB Froehler
Dec 1, 1990·The Journal of General Virology·K KonopkaN Düzgüneş
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L A YakubovV V Vlassov
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L LokeL M Neckers
May 1, 1993·AIDS Research and Human Retroviruses·M P SelvamJ S Epstein
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·G SchwabT Saison-Behmoaras
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M KriegC A Stein

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Citations

Sep 25, 1999·Journal of Cellular Physiology·U GalderisiA Giordano
Apr 7, 2004·Advanced Drug Delivery Reviews·Elias FattalCatherine Dubernet
May 18, 2000·Journal of Pharmaceutical and Biomedical Analysis·D ChenG S Srivatsa
Apr 3, 2013·Journal of Medicinal Chemistry·John F MillerAndrew J Peat
Jun 19, 2004·Anti-cancer Drugs·Cédric CastexJamal Temsamani
Jul 29, 2003·Journal of Pharmaceutical Sciences·Melanie A Lysik, Susanna Wu-Pong
Apr 20, 2006·Antiviral Research·Arthur Van Aerschot
Mar 17, 2007·Journal of Drug Targeting·José M LanaoClara I Colino
Dec 1, 2001·Expert Opinion on Biological Therapy·N DüzgünesM C Pedroso de Lima
Oct 13, 1998·Journal of Drug Targeting·A Désormeaux, M G Bergeron
Apr 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kathleen F PirolloEsther H Chang
Aug 28, 1997·Biochemical and Biophysical Research Communications·C Lavigne, A R Thierry

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