PMID: 6165634Apr 1, 1981Paper

Inhibition of hormone-induced cyclic AMP production and steroidogenesis in interstitial cells by deglycosylated lutropin

Molecular and Cellular Endocrinology
M R Sairam, P Fleshner

Abstract

We have investigated the ability of chemically deglycosylated ovine pituitary lutropin (DGLH) to stimulate cyclic AMP accumulation in rat interstitial cells in vitro. In sharp contrast to the native hormone which brought about a large increase in cyclic AMP levels, no significant response was indiced by DGLH even in the presence of the phosphodiesterase inhibitor, isobutyl methylxanthine. On the contrary, all preparations of DGLH tested were potent inhibitors of the action of the native hormone. The concentration of DGLH for inhibition of LH-induced cyclic AMP response was significantly lower than that required for inhibition of steroidogenesis. While inhibition of hormone-induced cyclic AMP response was complete, the inhibition of steroidogenesis in testicular cells by DGLH approached about 70-80%. Accumulation of cycli AMP induced by hCG was also effectively inhibited by DGLH suggesting that its inhibitory action is mediated via its binding to lutropin receptor(s) on interstitial cells. A recombinant of deglycosylated subunits, DG alpha + DG beta, had properties identical to that of DGLH.

References

Feb 3, 1975·Biochemical and Biophysical Research Communications·K SatoK Kosaka
Mar 1, 1975·Archives of Biochemistry and Biophysics·J Ramachandran, M R Sairam
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M L DufauK J Catt
Aug 1, 1979·International Journal of Peptide and Protein Research·M R Sairam
Oct 1, 1979·Archives of Biochemistry and Biophysics·M R Sairam, P W Schiller
Sep 1, 1976·Archives of Biochemistry and Biophysics·M R Sairam
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A G Gilman
Aug 15, 1973·Nature: New Biology·K J Catt, M L Dufau

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Citations

Oct 1, 1982·Molecular and Cellular Endocrinology·M R Sairam, P Manjunath
Oct 1, 1982·Molecular and Cellular Endocrinology·M R Sairam, P Manjunath
Sep 15, 2007·Reproductive Biomedicine Online·Roberto Palermo
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TakeuchiA Kobata
Jan 16, 2016·American Journal of Medical Genetics. Part a·Neta LoewenthalEli Hershkovitz
Feb 1, 1987·Baillière's Clinical Endocrinology and Metabolism·M L Dufau, J D Veldhuis
Jan 1, 1990·Endocrine Research·B A KeelS M Amir
Dec 1, 1985·Journal of Endocrinological Investigation·S AmrB D Weintraub
Mar 1, 1982·International Journal of Peptide and Protein Research·M R Sairam, P Manjunath
Dec 1, 1985·International Journal of Peptide and Protein Research·T A Bewley, M R Sairam
Jul 16, 1982·Biochimica Et Biophysica Acta·M R Sairam
Dec 17, 1985·Biochemical and Biophysical Research Communications·M I BermanS N Nayfeh
Nov 15, 1988·Biochemical and Biophysical Research Communications·W E Merz

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