PMID: 8995645Feb 1, 1997Paper

Inhibition of host cell transcription by poliovirus: cleavage of transcription factor CREB by poliovirus-encoded protease 3Cpro

Journal of Virology
P YalamanchiliA Dasgupta

Abstract

Host cell RNA polymerase II-mediated transcription is inhibited by poliovirus infection. Previous studies from our laboratory showed that activated transcription from a cyclic AMP-responsive element (CRE)-containing promoter was severely inhibited in extracts prepared from poliovirus-infected HeLa cells compared to those from mock-infected cells. Here we demonstrate that the CRE-binding protein, CREB, is specifically cleaved by the poliovirus-encoded protease 3Cpro both in vitro and in virus-infected cells. The proteolytic cleavage of CREB leads to a significant loss of its DNA binding as well as transcriptional activity. Additionally, we demonstrate that the phosphorylated, transcriptionally active form of CREB is cleaved by the viral protease in vitro. The results presented here suggest that a direct cleavage of CREB by the viral protease 3Cpro leads to inhibition of CREB-activated transcription in poliovirus-infected HeLa cells.

References

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Citations

Oct 12, 2000·Journal of Virology·R D Everett
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D S Lyles
Dec 31, 2011·The Journal of Infectious Diseases·Soo-Hyeon YunEun-Seok Jeon
Nov 8, 2012·MBio·Janet M RozovicsBert L Semler
Feb 6, 2016·Circulation Research·Gabriel FungBruce McManus
Mar 20, 2020·Microbiology and Molecular Biology Reviews : MMBR·Steven M PascalKomala Ponniah
Jun 22, 2007·Journal of Virology·Rushika PereraBert L Semler
Jan 22, 2010·The Journal of General Virology·Ksenia A KrupinaPeter V Lidsky
May 1, 1999·The Journal of Biological Chemistry·G A CoxQ M Wang
Jun 27, 2000·The Journal of Biological Chemistry·J SeipeltE Kuechler
Apr 23, 2005·The Journal of Biological Chemistry·Nickolay NeznanovAndrei V Gudkov
Sep 28, 2004·The Journal of General Virology·S P AminevaJ E Gern
Dec 22, 2010·Acta Biochimica Et Biophysica Sinica·Wenwu TianXianEn Zhang
Nov 28, 2007·Annual Review of Pathology·Mitra Esfandiarei, Bruce M McManus
May 5, 2016·Reviews in Medical Virology·Olli H LaitinenMalin Flodström-Tullberg
Dec 6, 2002·The Journal of General Virology·Tracey M HintonBrendan S Crabb
Apr 15, 2003·Journal of Virology·Pranav DanthiMarie Chow
Feb 28, 2006·Journal of Virology·Peter V LidskyVadim I Agol
Oct 1, 2011·Journal of Virology·James P White, Richard E Lloyd
Nov 20, 2015·Journal of Virology·Sabine M G van der SandenRalph A Tripp
Dec 6, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Stavroula FiliIrene Margiolaki
Aug 31, 2007·Journal of Virology·Rachel Groppo, Ann C Palmenberg

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