Abstract
Angiogenesis is a critical determinant of tumor growth and the development of metastases. Heparin, steroids, and heparin/steroid combinations have been used in a variety of in vitro models and in vivo in animal models as effective inhibitors of angiogenesis. We tested heparin, steroid and heparin/steroid combinations at a variety of concentrations to determine their effect on the human 'angiogenic switch' from a resting to a proliferative endothelium in vessels from three placentas (initiation), and the effect of these compounds on the subsequent growth of a human angiogenic response (promotion). Using full-thickness human placental vein discs cultured in three-dimensional fibrin-thrombin clots, we demonstrated that heparin (300, 3000 micrograms/ml), steroid (350, 3500 micrograms/ml), and combinations of heparin/steroid at these doses effectively blocked both initiation and promotion of a human angiogenic response in a dose-dependent fashion. We also demonstrated that high-dose steroid or heparin/steroid treatment for 15 days resulted in disruption of vessel integrity, while treatment with heparin alone produced a suppressed growth rate but had intact vessel architecture. High-dose heparin/steroid treatment could also disrupt a...Continue Reading
Citations
Mar 6, 2004·Journal of Thrombosis and Haemostasis : JTH·V KaushalP Mehta
Jul 5, 2007·Obesity Surgery·Frank L GreenwayEugene A Woltering
May 30, 2014·Experimental Biology and Medicine·Michael W IrvinAmbra Pozzi
Sep 24, 2004·International Journal of Experimental Pathology·Carolyn A StatonClaire E Lewis
Sep 25, 2003·Cancer Biotherapy & Radiopharmaceuticals·Eugene A Woltering
Jul 13, 2006·Phytotherapy Research : PTR·Zhijun LiuEugene A Woltering
Feb 14, 2007·Clinical Science·Renate T de JonghCoen D A Stehouwer
Jun 17, 2008·Human Reproduction Update·Anthony E Michael, Aris T Papageorghiou
Jun 16, 2007·In Vitro Cellular & Developmental Biology. Animal·Benedict Crabtree, Vasanta Subramanian
Jul 1, 2009·International Journal of Experimental Pathology·Carolyn A StatonNicola J Brown
Jul 3, 2008·Nature Clinical Practice. Gastroenterology & Hepatology·Inaam A Nakchbandi, J-Matthias Löhr
Jun 27, 2014·Neural Plasticity·Peter RotheneichnerSebastien Couillard-Despres
Feb 16, 2018·PloS One·Ruth MorganPatrick Hadoke
Jun 26, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Kouji HigashiYoichi Kohno
Oct 7, 2011·Advances in Therapy·Emma M TwinerFrank Greenway
Dec 31, 2003·Annals of Surgical Oncology·Seza A Gulec, Eugene A Woltering
Feb 3, 2005·Diabetes Technology & Therapeutics·Karl E Friedl
Jun 11, 2003·Annals of Surgery·Eugene A WolteringLynn H Harrison
Jul 1, 2017·Biomaterials Science·Shiyang ShenRan Mo
Sep 1, 2016·Biomacromolecules·Samantha J PaluckHeather D Maynard