PMID: 9420278Jan 7, 1998Paper

Inhibition of human cytomegalovirus DNA maturation by a benzimidazole ribonucleoside is mediated through the UL89 gene product

Journal of Virology
Mark R UnderwoodKaren K Biron

Abstract

2-Bromo-5,6-dichloro-1-beta-D-ribofuranosyl benzimidazole (BDCRB) is a member of a new class of benzimidazole ribonucleosides which inhibit human cytomegalovirus (HCMV) late in the replication cycle without inhibiting viral DNA synthesis. We show here that polygenomic concatemeric HCMV DNA does not mature to unit genome length in the presence of BDCRB. To discover the locus of action, virus resistant to BDCRB was selected by serial passage in the presence of the compound. Genetic mapping experiments with BDCRB-resistant virus demonstrated that the resistance phenotype mapped to one amino acid (Asp344Glu; low resistance) or two amino acids (Asp344Glu and Ala355Thr; high resistance) within the product of exon 2 of the HCMV U(L)89 open reading frame. The HCMV U(L)89 open reading frame and its homologs are among the most conserved open reading frames in the herpesviruses, and their products have sequence similarities to a known ATP-dependent endonuclease from the double-stranded DNA bacteriophage T4. These findings strongly suggest that BDCRB prevents viral DNA maturation by interacting with a U(L)89 gene product and that the U(L)89 open reading frame may encode an endonucleolytic subunit of the putative HCMV terminase. Further, si...Continue Reading

References

Jan 1, 1992·Virology·A J Davison
Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Oct 1, 1990·The Journal of General Virology·C AddisonV G Preston
Jan 1, 1989·Annual Review of Microbiology·L W Black
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M G DavisE S Huang
Aug 1, 1987·The Journal of General Virology·E S MocarskiR R Spaete
Sep 13, 1974·Nature·J King, S Casjens
Oct 18, 1972·Nature: New Biology·J D Watson
Sep 1, 1980·Antimicrobial Agents and Chemotherapy·K K Biron, G B Elion
Jan 1, 1984·Annual Review of Biochemistry·E H Blackburn
Jan 1, 1980·Antibiotics and Chemotherapy·P B Sehgal, I Tamm
Aug 1, 1994·AIDS Research and Human Retroviruses·M A Jacobson
Jan 1, 1994·Clinical Microbiology Reviews·A K Field, K K Biron

❮ Previous
Next ❯

Citations

Apr 20, 2002·Current Opinion in Infectious Diseases·Robert Snoeck, Erik De Clercq
Aug 16, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Nicolas J MuellerJay A Fishman
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·David L EversJohn C Drach
Oct 16, 2004·Expert Review of Anti-infective Therapy·Mark R Schleiss, Michael A McVoy
Jul 12, 2005·The Journal of Antimicrobial Chemotherapy·Edward Gershburg, Joseph S Pagano
Jul 26, 2008·Journal of Virology·Ritesh Tandon, Edward S Mocarski
Oct 24, 2008·Journal of Virology·Shelley K CockrellFred L Homa
Jan 6, 2010·Antimicrobial Agents and Chemotherapy·Peter LischkaHolger Zimmermann
Mar 3, 2015·Viruses·Ritesh TandonJames F Conway
Sep 25, 2015·Journal of Virology·Bernadette M DeRussy, Ritesh Tandon
Nov 26, 1999·Antiviral Chemistry & Chemotherapy·A K Field
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D G WolfE S Mocarski
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Dean W SellesethRobert J Harvey
Sep 7, 2002·Drugs·Vincent C Emery, Aycan F Hassan-Walker
Apr 6, 2004·FEBS Letters·Christos G W SavvaElke Bogner
Mar 7, 2020·The Journal of Infectious Diseases·Edward AcostaRichard Whitley
May 19, 2000·Antimicrobial Agents and Chemotherapy·M MarschallT Stamminger
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·Mark R UnderwoodF Leslie Boyd
Mar 28, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Philip L LorenziGordon L Amidon
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·Earl R KernDeborah J Bidanset
May 20, 2005·The Journal of Pharmacology and Experimental Therapeutics·Philip L LorenziGordon L Amidon
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·J J McSharryG L Drusano
Jul 31, 2009·Journal of Virology·Ritesh TandonEdward S Mocarski
Oct 22, 2008·The Journal of General Virology·Jian Ben Wang, Michael A McVoy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.