Inhibition of human immunodeficiency virus replication and growth advantage of CD4+ T cells from HIV-infected individuals that express intracellular antibodies against HIV-1 gp120 or Tat

Human Gene Therapy
M C PoznanskyW A Marasco

Abstract

Current clinical gene therapy protocols for the treatment of human immunodeficiency virus type 1 (HIV-1) infection often involve the ex vivo transduction and expansion of CD4+ T cells derived from HIV-positive patients at a late stage in their disease (CD4 count <400). These protocols involve the transduction of T cells by murine leukemia virus (MLV)-based vectors encoding antiviral constructs such as the rev m10 dominant negative mutant or a ribozyme directed against the CAP site of HIV-1 RNA. We examined the efficiency and stability of transduction of CD4+ T cells derived from HIV-infected patients at different stages in the progression of their disease, from seroconversion to AIDS. CD4+ T cells from HIV-positive patients and uninfected donors were transduced with MLV-based vectors encoding beta-galactosidase and an intracellular antibody directed against gp120 (sFv 105) or Tat. (sFvtat1-Ckappa). The expression of marker genes and the effects of the antiviral constructs were monitored in vitro in unselected transduced CD4+ T cells. Efficiency and stability of transduction varied during the course of HIV infection; CD4+ T cells derived from asymptomatic patients were transducible at higher efficiencies and stabilities than CD4...Continue Reading

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Citations

Jan 29, 2000·Journal of Immunological Methods·W A MarascoA Winkler
Jul 8, 1999·Human Gene Therapy·F Paillard
Feb 13, 2002·AIDS Patient Care and STDs·Mario R Mautino, Richard A Morgan
Aug 7, 1998·Human Gene Therapy·W A MarascoS D Jones
Nov 29, 2008·Journal of Immunological Methods·N StrebeS Dübel
Jan 31, 2004·Annals of the New York Academy of Sciences·Alessandro MichienziJohn J Rossi
Aug 8, 2015·MAbs·Andrea L J MarschallThomas Böldicke
Aug 29, 2003·Expert Opinion on Biological Therapy·Ananthalakshmi PoluriRichard E Sutton
Sep 24, 1999·Biochemical and Biophysical Research Communications·T HeintgesJ R Wands
Mar 16, 2002·Gene Therapy·G PalùF Manca
Aug 2, 2017·Advanced Pharmaceutical Bulletin·Mohammad Tasyriq Che Omar
Aug 15, 2003·The Journal of General Virology·Olga ArtsaenkoTobias Heintges
Apr 18, 2020·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Congcong ZhangAndrea L J Marschall

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