PMID: 6397595Nov 1, 1984Paper

Inhibition of human NK cell cytotoxicity against K562 cells with glycopeptides from K562 plasma membranes

Journal of Clinical & Laboratory Immunology
J M DeckerE W Ades

Abstract

Human Natural Killer (NK) cell activity against K562 target cells has previously been shown to be inhibited by certain monosaccharides and glycoproteins in a specific fashion. Glycopeptides have been prepared from the isolated plasma membranes of cultured K562 cells by extensive pronase digestion and fractionated by lectin affinity chromatography. Nine different fractions were tested for their ability to block 51Cr release by K562 targets in the presence of NK cells; two of the fractions, one isolated on RCA I lectin and the second on Pea lectin, were very effective at blocking cytotoxicity (greater than 80% inhibition) while the other fractions were only marginally effective. Furthermore, evidence is presented which demonstrates the blocking activity of the glycopeptides occurred at a lytic rather than a recognition step.

Related Concepts

Related Feeds

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.