PMID: 11334960May 4, 2001Paper

Inhibition of human NK cell-mediated cytotoxicity by exposure to ammonium chloride

Journal of Immunological Methods
C BranderJ D Seebach

Abstract

Ammonium-chloride-containing solutions (AC) are routinely used to lyse red blood cells during preparation of PBMC. Although exposure to AC has been described to affect the ultrastructural appearance of large granular lymphocytes and to temporarily inhibit cytolytic activity of PBMC preparations, the cellular basis of this phenomenon has not been studied. Here, the inhibitory effect of AC on human CTL and NK-mediated cytotoxicity has been analyzed in 4-h 51Cr-release assays. The results show that NK killing of K562 leukemia cells and xenogeneic endothelial cells is inhibited by AC exposure. The effect is dose-dependent and reversible, because recovery of cytotoxicity is observed within 15 h of re-culturing. AC does not reduce the viability of NK cells and the inhibitory effect is not mediated by the exhaustive release of granzymes upon AC treatment. In contrast, antigen-specific CTL killing of EBV-transformed B-lymphoblastoid cell lines and xenogeneic PHA lymphoblasts was less sensitive to AC and data are presented suggesting that FasL-induced apoptosis is not inhibited by AC. In conclusion, perforin-mediated NK killing is AC-sensitive whereas CTL killing and FasL-mediated killing appear to be AC-resistant. Therefore, AC represe...Continue Reading

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Citations

May 3, 2003·Free Radical Biology & Medicine·Zhengquan YuUlf T Brunk
Aug 21, 2010·Xenotransplantation·Sarah B KennettEda T Bloom
Feb 8, 2018·Journal of Immunology Research·Gisella Puga YungJörg D Seebach
Jan 27, 2010·International Reviews of Immunology·Fang Zhou
Apr 30, 2021·Scandinavian Journal of Immunology·Jiahui XuTing Li

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