Inhibition of human periodontal prostaglandin E2 synthesis with selected agents

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S OffenbacherF J Sena

Abstract

Considerable evidence has demonstrated the importance of PGE2 synthesis in the pathogenesis of periodontal disease. Although various cyclooxygenase inhibitors have been known to block periodontal PGE2 synthesis and prevent disease progression in animal models, there are few reports comparing relative efficacies of various inhibitors of arachidonic acid (ARA) metabolism. We have developed a sensitive in vitro assay to measure PGE2 synthesis in periodontal tissues. The apparent IC50 values (i.e. the concentration of drug which causes 50% inhibition of maximum PGE2 synthesis) have been determined for a series of arachidonic acid analogues as well as competitive and non-competitive cyclooxygenase inhibitors. Periodontal tissue homogenates were incubated in the presence of 3H-arachidonic acid for 45 min at 37 degrees C. Inhibitors were tested at 10(-10)-10(-4) M and at zero concentration to measure conversion of 3H-arachidonate to 3H-PGE2. Log or half log dilutions of inhibitors were tested in triplicate for each assay. Radiolabeled PGE2 was extracted from homogenates, purified by reverse phase chromatography and quantitated by double antibody capture. RIA was performed on each homogenate to determine the amount of endogenous unlabe...Continue Reading

References

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Citations

Apr 26, 2003·Nutrition·Christine S Ritchie, Denis F Kinane
Feb 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Elliot D RosensteinGregory Kazandjian
Nov 1, 1993·Journal of Clinical Periodontology·T B Taiyeb Ali, I M Waite
Nov 1, 1993·Journal of Clinical Periodontology·P A HeasmanR A Seymour
Aug 1, 1997·Journal of Clinical Periodontology·D W PaquetteR C Williams
Jan 1, 1997·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·B E RingdahlC C Organ
Nov 1, 1994·Journal of Periodontal Research·G K JohnsonS D Vincent
Nov 1, 1996·Journal of Periodontal Research·K L LiS Offenbacher
Jan 21, 2011·Journal of Dental Research·M V Thomas, D A Puleo
Nov 1, 1996·Annals of Periodontology·C H Drisko
Jul 1, 2009·Journal of Periodontology·Hatice HasturkAlpdogan Kantarci
Feb 13, 2009·Journal of Periodontal Research·A BendykP M Bartold
Nov 26, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Amr M El Khouli, Enas Ahmed El-Gendy
Nov 21, 1998·Journal of Dental Research·H P LawrenceS Offenbacher
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·T H Howell, R C Williams
Sep 15, 1998·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·G K JohnsonS L Slager

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