Inhibition of human prostate cancer (PC-3) cells and targeting of PC-3-derived prostate cancer stem cells with koenimbin, a natural dietary compound from Murraya koenigii (L) Spreng

Drug Design, Development and Therapy
Behnam KamalidehghanFatemeh Ahmadipour

Abstract

Inhibition of prostate cancer stem cells (PCSCs) is an efficient curative maintenance protocol for the prevention of prostate cancer. The objectives of this study were to assess the efficiency of koenimbin, a major biologically active component of Murraya koenigii (L) Spreng, in the suppression of PC-3 cells and to target PC-3-derived cancer stem cells (CSCs) through apoptotic and CSC signaling pathways in vitro. The antiproliferative activity of koenimbin was examined using MTT, and the apoptotic detection was carried out by acridine orange/propidium iodide (AO/PI) double-staining and multiparametric high-content screening (HCS) assays. Caspase bioluminescence assay, reverse transcription polymerase chain reaction (RT-PCR), and immunoblotting were conducted to confirm the expression of apoptotic-associated proteins. Cell cycle analysis was investigated using flow cytometry. Involvement of nuclear factor-kappa B (NF-κB) was analyzed using HCS assay. Aldefluor™ and prostasphere formation examinations were used to evaluate the impact of koenimbin on PC-3 CSCs in vitro. Koenimbin remarkably inhibited cell proliferation in a dose-dependent manner. Koenimbin induced nuclear condensation, formation of apoptotic bodies, and G0/G1 phas...Continue Reading

Citations

Jun 26, 2020·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Sarvnarinder KaurAniqa Aniqa
Mar 3, 2020·Human Antibodies·Maliheh AstanehElahe Motevaseli
May 12, 2020·Frontiers in Cell and Developmental Biology·Md Ataur RahmanSsang-Goo Cho
Jul 6, 2019·Anti-cancer Agents in Medicinal Chemistry·Plabon K DasFarhadul Islam
Nov 22, 2019·Anti-cancer Agents in Medicinal Chemistry·Rumana AhmadArvind K Srivastava
Jun 20, 2020·Drug Discovery Today·Priyanka MohapatraSanjeeb Kumar Sahoo
Nov 23, 2021·Journal of the American Chemical Society·Elizabeth M BolithoPeter J Sadler

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Methods Mentioned

BETA
enzymatic assay
fluorescence microscopy
PCR
profiler
electrophoresis
protein array

Software Mentioned

Statistical Package for Social Sciences
ImageJ
Floro
CellQuest Pro
BD
MetaMorph

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