Inhibition of hyphae formation and SIR2 expression in Candida albicans treated with fresh Allium sativum (garlic) extract

Journal of Applied Microbiology
C F LowFauziah Othman

Abstract

The aims of the present study were to determine whether Allium sativum (garlic) extract has any effect on the morphology transformation of Candida albicans, and to investigate whether it could alter the gene expression level of SIR2, a morphogenetic control gene and SAP4, a gene encoding secreted aspartyl proteinase. Candida albicans cells were incubated with a range of concentrations of fresh garlic extract, and the morphology was monitored via light microscopy. Garlic extract treatment caused the transition of yeast form to hyphal form to be obviated. The expression of SIR2 was down-regulated from 1.2- to 2.5-fold with increasing concentration of the garlic extract, as determined from relative quantitative reverse transcription-polymerase chain reaction. There was no difference in the SAP4 expression in control vs treated cultures. Garlic and its bioactive components have the ability to suppress hyphae production and to affect the expression level of SIR2 gene. Hyphal production is an essential virulence determinant of C. albicans for invasive infections, therefore garlic and its constituents can be effective not only against colonizing C. albicans strains present in mucosal infections, but also virulent strains causing syste...Continue Reading

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Citations

Jun 7, 2011·Eukaryotic Cell·Julie Shareck, Pierre Belhumeur
Jan 19, 2016·Frontiers in Physiology·Lisa C Barros de Andrade E SousaEdda Klipp
Feb 24, 2016·Pharmaceutical Patent Analyst·Sukhbir K Shahid
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Dec 7, 2013·BJOG : an International Journal of Obstetrics and Gynaecology·C J WatsonM Pirotta
Jun 11, 2015·Jundishapur Journal of Microbiology·Guoliang LiChaofeng Sun
Oct 30, 2015·Singapore Medical Journal·Farzaneh EbrahimyHamid Alavi Majd
Mar 31, 2016·PLoS Neglected Tropical Diseases·María J CorralJosé M Alunda
Nov 15, 2016·MSphere·Pim T van LeeuwenBastiaan P Krom
Aug 23, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Gamal A ElmowalidAdel M Attia
Aug 6, 2020·Current Drug Targets·Graziela Vargas Rigo, Tiana Tasca

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