Inhibition of hypoxia-inducible factor-1 alpha radiosensitized MG-63 human osteosarcoma cells in vitro

Tumori
Zhu JinHu Xiang

Abstract

Hypoxia is a fundamental microenvironmental component of osteosarcoma which induces activation of the hypoxia-inducible factor-1 (HIF-1) pathway. Overexpression of HIF-1α has been linked to tumor resistance to radio- or chemotherapy. However, little is known about the effects of HIF-1α inhibition on hypoxic radioresistance of human osteosarcoma cells. Here, we investigated the effects of HIF-1α inhibition on cell survival and radiosensitivity in the MG-63 human osteosarcoma cell line. HIF-1α inhibition was achieved by small interfering RNA (siRNA) targeting of HIF-1α or via chetomin. Inhibition of the HIF-1 pathway was determined by monitoring the expression levels of HIF-1α, carbonic anhydrase 9 (CA9) and vascular endothelial growth factor (VEGF) using quantitative real-time PCR and Western blot analyses. Clonogenic assay was performed after irradiation (2-10 Gy) to investigate the effect of HIF-1α inhibition on the radiosensitivity of human osteosarcoma cells under normoxic and hypoxic conditions. Compared to the control groups, treatment with HIF-1α siRNA or chetomin significantly reduced the hypoxia-inducible radioresistance of MG-63 cells. However, siRNA and chetomin showed different effects on the radiosensitivity under n...Continue Reading

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Citations

Oct 28, 2016·Advanced Drug Delivery Reviews·Sergio ReyBradly G Wouters
Sep 4, 2020·Cells·Marina PierrevelcinNatacha Entz-Werlé
Jan 6, 2021·Cancers·Zachary D Prudowsky, Jason T Yustein
Jun 23, 2021·Journal of Experimental & Clinical Cancer Research : CR·Irma TelarovicMartin Pruschy
Aug 8, 2021·Cancers·Gemma Di PompoSofia Avnet
Dec 11, 2021·Journal of Magnetic Resonance Imaging : JMRI·Chu Hyun KimSang-Hee Choi

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Methods Mentioned

BETA
transfection
PCR
electrophoresis
dissect

Software Mentioned

GraphPad Prism
SPSS
GraphPad

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